Anti-Prostate Specific Antigen 抗体 (ab53774)
Key features and details
- Rabbit polyclonal to Prostate Specific Antigen
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Prostate Specific Antigen antibody
Prostate Specific Antigen 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Prostate Specific Antigen -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from human Prostate Specific Antigen
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
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| ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 354 Human
- Entrez Gene: 16621 Mouse
- Omim: 176820 Human
- SwissProt: P07288 Human
- SwissProt: P00756 Mouse
- Unigene: 171995 Human
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別名
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
画像
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Western blot - Anti-Prostate Specific Antigen antibody (ab53774)All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution
Lane 1 : Extracts from mouse heart
Lane 2 : Extracts from mouse heart, plus immunising peptide
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab53774 は 15 報の論文で使用されています。
- Wissmiller K et al. Inceptor correlates with markers of prostate cancer progression and modulates insulin/IGF1 signaling and cancer cell migration. Mol Metab 71:101706 (2023). PubMed: 36931467
- Shin WS et al. Pinostilbene inhibits full-length and splice variant of androgen receptor in prostate cancer. Sci Rep 13:16663 (2023). PubMed: 37794090
- Whitburn J et al. Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival. Sci Adv 8:eabf9096 (2022). PubMed: 35213227
- Wang H et al. Lncap-AI prostate cancer cell line establishment by Flutamide and androgen-free environment to promote cell adherent. BMC Mol Cell Biol 23:51 (2022). PubMed: 36443669
- Franko A et al. Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study. Genes (Basel) 11:N/A (2020). PubMed: 33036464