Anti-Prostate Specific Antigen 抗体 [A67-B/E3] (ab2218)
Key features and details
- Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Anti-Prostate Specific Antigen antibody [A67-B/E3]
Prostate Specific Antigen 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen -
由来種
Mouse -
特異性
This antibody is specific for PSA. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Prostate Specific Antigen. 30 kDa protein fraction prepared from human seminal plasma
-
ポジティブ・コントロール
- WB: Human seminal plasma. IHC-P: Human prostate tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein A purified -
特記事項(精製)
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
A67-B/E3 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2218の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 29 kDa.
|
|
IHC-P |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 29 kDa. |
IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
-
別名
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
画像
-
All lanes : Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218) at 5 µg/ml
All lanes : Human Seminal Plasma
Secondary
All lanes : IRDye800-conjugated Anti-Mouse IgG1 Secondary Antibody
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218)
Immunohistochemical analysis of human prostate tissue sections staining Prostate Specific Antigen with ab2218.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab2218 は 1 報の論文で使用されています。
- Rye P.D. (eds.) et al. Summary Report on the ISOBM TD-3 Workshop. Tum. Biol. 20:1-94 (1999). PubMed: