Anti-Prostaglandin E Synthase/MPGES-1 抗体 [EPR13765] (ab180589)
![Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)](https://www.abcam.co.jp/ps/products/180/ab180589/Images/ab180589-211280-anti-prostaglandin-e-synthase-mpges-1-antibody-epr13765-western-blot.jpg "Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13765] to Prostaglandin E Synthase/MPGES-1
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]
Prostaglandin E Synthase/MPGES-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13765] to Prostaglandin E Synthase/MPGES-1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- NIH 3T3, Jurkat and A549 cell line lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 0.05% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13765 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180589の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa). |
ターゲット情報
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機能
Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). -
配列類似性
Belongs to the MAPEG family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9536 Human
- Entrez Gene: 64292 Mouse
- Entrez Gene: 59103 Rat
- Omim: 605172 Human
- SwissProt: O14684 Human
- SwissProt: Q9JM51 Mouse
- Unigene: 146688 Human
- Unigene: 28768 Mouse
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別名
- Glutathione S transferase 1 like 1 antibody
- MGC10317 antibody
- MGST IV antibody
see all
画像
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Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)All lanes : Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution
Lane 1 : Lysate of NIH 3T3 cells with Immunizing peptide
Lane 2 : Lysate of NIH 3T3 cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)](https://www.abcam.co.jp/ps/products/180/ab180589/Images/ab180589-211279-anti-prostaglandin-e-synthase-mpges-1-antibody-epr13765-western-blot.jpg)
Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)All lanes : Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution
Lane 1 : NIH 3T3 cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : HeLa cell line lysate
Lane 4 : A549 cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
![Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)](https://www.abcam.co.jp/ps/products/180/ab180589/Images/ab180589-4-benefits-of-recombinant-antibodies.png)
Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab180589 は 7 報の論文で使用されています。
- Kojima F et al. Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate-induced colitis in mice lacking microsomal prostaglandin E synthase-1. Inflamm Regen 42:1 (2022). PubMed: 34983695
- Hagiwara M et al. MUC1-C integrates type II interferon and chromatin remodeling pathways in immunosuppression of prostate cancer. Oncoimmunology 11:2029298 (2022). PubMed: 35127252
- Miek L et al. Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid. Immunology 166:47-67 (2022). PubMed: 35143048
- Monteleone NJ & Lutz CS miR-708 Negatively Regulates TNFa/IL-1ß Signaling by Suppressing NF-?B and Arachidonic Acid Pathways. Mediators Inflamm 2021:5595520 (2021). PubMed: 33776573
- Monteleone NJ & Lutz CS miR-708-5p targets oncogenic prostaglandin E2 production to suppress a pro-tumorigenic phenotype in lung cancer cells. Oncotarget 11:2464-2483 (2020). PubMed: 32655834
- Monteleone NJ & Lutz CS miR-708-5p enhances erlotinib/paclitaxel efficacy and overcomes chemoresistance in lung cancer cells. Oncotarget 11:4699-4721 (2020). PubMed: 33473256
- Bogdan D et al. Fatty acid-binding protein 5 controls microsomal prostaglandin E synthase 1 (mPGES-1) induction during inflammation. J Biol Chem 293:5295-5306 (2018). PubMed: 29440395