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リコンビナントRabMAb

Anti-proNGF 抗体 [EP1318Y] (ab68151)

Submit a review Submit a question References (4)
Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (ab68151)
  • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
  • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
  • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
  • Anti-proNGF antibody [EP1318Y] (ab68151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1318Y] to proNGF
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-proNGF antibody [EP1318Y]
    proNGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1318Y] to proNGF

  • 由来種

    Rabbit

  • 特異性

    This antibody recognizes pro-NGF and can work in tissues rather than cell lines for WB.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and Rat brain lysates. Mouse, Rat and Human cerebellum lysates. Human pro-NGF recombinant protein lysates. Flow Cyt (Intra): SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68151の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200 - 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 27 kDa).

For unpurified use at 1/1000 - 1/5000.
特記事項
Flow Cyt (Intra)
1/200 - 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 27 kDa).

For unpurified use at 1/1000 - 1/5000.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).

  • 関連疾患

    Neuropathy, hereditary sensory and autonomic, 5

  • 配列類似性

    Belongs to the NGF-beta family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • Precursor nerve growth factor antibody
      • proNGF antibody
      • proNGF_HUMAN antibody
      • proNGFB antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (ab68151)
      Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (ab68151)

      Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line) 

       

       

    • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      All lanes : Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Human cerebellum lysate
      Lane 4 : Mouse cerebellum lysate
      Lane 5 : Rat cerebellum lysate
      Lane 6 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 27 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Compared with ab68151, ab52918 has a higher affinity in positive samples.

      The positive cell lines (Lanes 7-9) are consistent with the literature: PMID:12944907, Fig1.

      Blocking buffer: 5% NFDM/TBST.

    • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 27 kDa

    • Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      Western blot - Anti-proNGF antibody [EP1318Y] (ab68151)
      All lanes : Anti-proNGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

      Lane 1 : 10ng human pro-NGF recombinant protein
      Lane 2 : 5ng human pro-NGF recombinant protein
      Lane 3 : 1ng human pro-NGF recombinant protein
      Lane 4 : 0.5ng human pro-NGF recombinant protein

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa
      Observed band size: 27 kDa
      Additional bands at: 13 kDa, 20 kDa. We are unsure as to the identity of these extra bands.

    • Anti-proNGF antibody [EP1318Y] (ab68151)
      Anti-proNGF antibody [EP1318Y] (ab68151)

    参考文献 (4)

    ab68151 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68151 は 4 報の論文で使用されています。

    • Sun NY  et al. Astragaloside-IV modulates NGF-induced osteoblast differentiation via the GSK3β/β-catenin signalling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33179111
    • Piovesana R  et al. Muscarinic receptors modulate Nerve Growth Factor production in rat Schwann-like adipose-derived stem cells and in Schwann cells. Sci Rep 10:7159 (2020). PubMed: 32346125
    • Protto V  et al. Electroacupuncture in rats normalizes the diabetes-induced alterations in the septo-hippocampal cholinergic system. Hippocampus N/A:N/A (2019). PubMed: 30870587
    • Cai H  et al. Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-ß in APP/PS1 mice. Oncotarget 8:92621-92634 (2017). PubMed: 29190943

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