AB68151

Anti-proNGF 抗体 [EP1318Y]

Anti-proNGF antibody [EP1318Y]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal NGF antibody. Propeptide domain. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse, Recombinant full length protein - Human samples. Cited in 4 publications.

別名を表示する

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

4 Images

Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (AB68151)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (AB68151)

Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

Supplier Data

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

Compared with ab68151, ab52918 has a higher affinity in positive samples.

The positive cell lines (Lanes 7-9) are consistent with the literature : PMID : 12944907, Fig1.

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

Human cerebellum lysate at 20 µg

Lane 4:

Mouse cerebellum lysate at 20 µg

Lane 5:

Rat cerebellum lysate at 20 µg

Lane 6:

Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 30 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

All lanes:

Western blot - Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 27 kDa

false

Unknown

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

All lanes:

Western blot - Anti-proNGF antibody [EP1318Y] (ab68151) at 1/5000 dilution

Lane 1:

10ng human pro-NGF recombinant protein

Lane 2:

5ng human pro-NGF recombinant protein

Lane 3:

1ng human pro-NGF recombinant protein

Lane 4:

0.5ng human pro-NGF recombinant protein

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 13 kDa,130 kDa,20 kDa,27 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1318Y

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Not suitable for testing cell lines in WB.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/5000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200 - 1/280", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/5000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/5000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/5000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as proNGF also referred to as precursor nerve growth factor is the biological precursor of mature NGF. ProNGF is significantly larger than NGF with a molecular weight of approximately 35 kDa. It is mainly expressed in the nervous system including the brain and peripheral tissues. The synthesis of proNGF occurs in various types of neurons and glial cells and it serves as a precursor that undergoes proteolytic cleavage to become the biologically active form NGF.

Biological function summary

ProNGF plays a role in modulating neuronal survival growth and differentiation. As a part of a larger signaling complex it interacts with receptors such as p75NTR and sortilin which influence the fate of neurons. Unlike its mature form proNGF tends to be associated with inducing apoptosis under certain conditions. The balance between proNGF and NGF levels in tissues can affect neural health and function significantly.

Pathways

The interactions of proNGF impact key neurological pathways. It has been implicated in the JNK signaling pathway which is important for stress response and apoptosis. ProNGF also relates to the PI3K/Akt pathway due to its interaction partners influencing cell survival and growth. Receptor binding with proteins such as TrkA and p75NTR directly affects downstream signaling cascades integrating proNGF into broader neurotrophic signaling networks.

Imbalances in proNGF activity relate to the progression of neurodegenerative conditions like Alzheimer's disease where it may contribute to abnormal apoptosis in the brain. Studies also suggest its involvement in peripheral neuropathies where changes in proNGF levels lead to altered nerve function. ProNGF along with related proteins like sortilin and p75NTR could serve as potential biomarkers or therapeutic targets in managing such conditions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

See full target information NGF

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33179111

2020

Astragaloside‑IV modulates NGF‑induced osteoblast differentiation via the GSK3β/β‑catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Yang Sun,Xiao-Lan Liu,Juan Gao,Xiao-Hui Wu,Ben Dou

Scientific reports 10:7159 PubMed32346125

2020

Muscarinic receptors modulate Nerve Growth Factor production in rat Schwann-like adipose-derived stem cells and in Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

R Piovesana,A Faroni,M Taggi,A Matera,M Soligo,R Canipari,L Manni,A J Reid,A M Tata

Hippocampus 29:891-904 PubMed30870587

2019

Electroacupuncture in rats normalizes the diabetes-induced alterations in the septo-hippocampal cholinergic system.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Protto,Marzia Soligo,Maria Egle De Stefano,Stefano Farioli-Vecchioli,Lionel N J L Marlier,Robert Nisticò,Luigi Manni

Oncotarget 8:92621-92634 PubMed29190943

2017

Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-β in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haobin Cai,Yijie Wang,Jiayang He,Tiantian Cai,Jun Wu,Jiansong Fang,Rong Zhang,Zhouke Guo,Li Guan,Qinkai Zhan,Li Lin,Yao Xiao,Huafeng Pan,Qi Wang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-proNGF 抗体 [EP1318Y]

Anti-proNGF antibody [EP1318Y]

Flow Cytometry (Intracellular) - Anti-proNGF antibody [EP1318Y] (AB68151)

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

Western blot - Anti-proNGF antibody [EP1318Y] (AB68151)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise