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リコンビナントRabMAb

Anti-Prolactin/PRL 抗体 [EPR19386] (ab183967)

Submit a review Submit a question References (3)
Western blot - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
  • Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19386] to Prolactin/PRL
  • Suitable for: WB, IHC-Fr, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Prolactin/PRL antibody [EPR19386]
    Prolactin/PRL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19386] to Prolactin/PRL

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Fr, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and rat pituitary lysates. IHC-P: Mouse adenohypophysis tissue. IHC-Fr: Mouse pituitary tissue. ICC/IF: HeLa cells transfected with Myc tagged Prolactin/PRL.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19386

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183967の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr
1/200.

Perform antigen retrieval with heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
特記事項
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr
1/200.

Perform antigen retrieval with heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

ターゲット情報

  • 関連性

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Decidual prolactin antibody
      • GHA1 antibody
      • Growth hormone A1 antibody
      • Lactogenic hormone antibody
      • Luteotropic hormone antibody
      • Mammotropin antibody
      • PRL antibody
      • Prolactin antibody
      • Prolactin precursor antibody
      see all

    画像

    • Western blot - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Western blot - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      All lanes : Anti-Prolactin/PRL antibody [EPR19386] (ab183967) at 1/1000 dilution

      Lane 1 : Mouse pituitary lysate
      Lane 2 : Mouse uterus lysate
      Lane 3 : Rat heart lysate
      Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 6 : Rat pituitary lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1, 2, 3, 4 and 5: 5 seconds; Lane 6: 1 second.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunohistochemical analysis of paraffin-embedded Mouse adenohypophysis tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse adenohypophysis was observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

       

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining was observed on mouse spleen.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining was observed on mouse stomach.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183967 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      The result showed cytoplasmic staining on mouse anterior pituitary. 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183967 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      No staining was observed on mouse kidney. 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Prolactin/PRL with ab183967 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      The results show positive staining on HeLa cells transfected with myc-tagged Prolactin.

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab183967 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
      -ve control 2:  ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Prolactin/PRL with ab183967 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      The results show positive staining on HeLa cells transfected with myc-tagged Prolactin.

      The nuclear counter stain is DAPI (blue).

      Myc is detected with anti-c-Myc (mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab183967 at 1/100 dilution followed by ab150120  at 1/1000 dilution.
      -ve control 2: Anti-c-Myc  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    • Anti-Prolactin/PRL antibody [EPR19386] (ab183967)
      Anti-Prolactin/PRL antibody [EPR19386] (ab183967)

    参考文献 (3)

    ab183967 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183967 は 3 報の論文で使用されています。

    • Zhao GK  et al. TRH Regulates the Synthesis and Secretion of Prolactin in Rats with Adenohypophysis through the Differential Expression of miR-126a-5p. Int J Mol Sci 23:N/A (2022). PubMed: 36555554
    • Wang L  et al. MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-ß-Treated Endometrial Stromal Cells via the MAPK and Wnt/ß-Catenin Signaling Pathways. Pathol Oncol Res 27:1609761 (2021). PubMed: 34257616
    • Guernsey MW  et al. Molecular Signatures of Placentation and Secretion Uncovered in Poeciliopsis Maternal Follicles. Mol Biol Evol 37:2679-2690 (2020). PubMed: 32421768

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