Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Prolactin/PRL 抗体 [EPR19372] (ab183968)

Submit a review Submit a question
Western blot - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
  • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
  • Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19372] to Prolactin/PRL
  • Suitable for: IHC-Fr, WB, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Prolactin/PRL antibody [EPR19372]
    Prolactin/PRL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19372] to Prolactin/PRL

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and rat pituitary lysates. IHC-Fr: Mouse pituitary tissue. IP: Mouse pituitary whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19372

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183968の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/200.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

This application is recommended for mouse only.
WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP
1/30.
特記事項
IHC-Fr
1/200.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

This application is recommended for mouse only.
WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP
1/30.

ターゲット情報

  • 関連性

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Decidual prolactin antibody
      • GHA1 antibody
      • Growth hormone A1 antibody
      • Lactogenic hormone antibody
      • Luteotropic hormone antibody
      • Mammotropin antibody
      • PRL antibody
      • Prolactin antibody
      • Prolactin precursor antibody
      see all

    画像

    • Western blot - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      Western blot - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      All lanes : Anti-Prolactin/PRL antibody [EPR19372] (ab183968) at 1/5000 dilution

      Lane 1 : Mouse pituitary lysate
      Lane 2 : Rat pituitary lysate
      Lane 3 : Mouse uterus tissue lysate
      Lane 4 : Rat kidney tissue lysate
      Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 8 seconds; Lane 2,3,4,5 and  6: 3 second.

    • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      The result showed cytoplasmic staining on mouse anterior pituitary. 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      The result showed negative staining on mouse kidney. 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

      Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary whole cell lysate with ab183968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183968 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse pituitary whole cell lysate,  10µg (Input).

      Lane 2: ab183968 IP in Mouse pituitary whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183968 in Mouse pituitary whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-Prolactin/PRL antibody [EPR19372] (ab183968)
      Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

    参考文献 (0)

    ab183968 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183968 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab183968.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com