AB154589

Anti-Prohibitin 抗体

Name: Anti-Prohibitin 抗体 (ab154589) | Abcam
Brand: Abcam Limited
SKU: AB154589
Price: 78000 JPY
Availability: InStock

Anti-Prohibitin antibody

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(2 Publications)

Rabbit Polyclonal Prohibitin antibody. Suitable for IP, IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHB1 aa 1-300.

別名を表示する

PHB, PHB1, Prohibitin 1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemical analysis of paraffin-embedded Human OVCAR3 xenograft tissue labeling Prohibitin with ab154589 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (AB154589)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (AB154589)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labeling Prohibitin with ab154589 at 1/500 dilution (Green). Alpha-tubulin filaments are labeled in red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (AB154589)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Prohibitin protein at mitochondria using ab154589 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker stained by anti-phalloidin antibody at a 1/100 dilution (Red). Hoechst 33342 was used for nuclear staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for Prohibitin protein at mitochondria with ab154589 at a 1/500 dilution.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemical analysis of paraffin-embedded rat liver tissue stained for Prohibitin protein at cytoplasm with ab154589 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue stained for Prohibitin protein at cytoplasm with ab154589 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Unknown

Western blot - Anti-Prohibitin antibody (AB154589)

12% SDS PAGE

All lanes:

Western blot - Anti-Prohibitin antibody (ab154589) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 24 kDa,28 kDa,30 kDa,35 kDa

false

Supplier Data

Western blot - Anti-Prohibitin antibody (AB154589)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Prohibitin antibody (ab154589) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 30 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IP, ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PHB1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P35232

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prohibitin often known as PHB is a 30 kDa protein that plays significant mechanical roles in cellular processes. It is widely present in various tissues and cells acting as a mitochondrial marker and associates with other mitochondrial markers. Prohibitin protein exists in a highly conserved structure which makes it essential across different species. It localizes within the inner mitochondrial membrane where it exerts functions important for cellular energy homeostasis and mitochondrial stability.

Biological function summary

The protein is involved in the regulation of mitochondrial respiratory function and stabilization of mitochondrial nucleoid. Prohibitin protein forms a complex with related proteins contributing to the maintenance of mitochondrial morphology and integrity. Its role extends to cell cycle control and apoptosis making it a significant player in cellular proliferation and survival. Additionally it influences cellular senescence and stress responses offering protection against various stressors.

Pathways

Prohibitin integrates into mitochondrial dynamics and energy production pathways. It participates in the regulation of oxidative phosphorylation a core component for ATP generation. Prohibitin collaborates with proteins like co-chaperones and complexes such as the inner mitochondrial membrane complex which further illustrates its integration into cellular pathways. The interplay of these proteins and pathways underlines Prohibitin's role in maintaining mitochondrial function.

Prohibitin shows notable connections to cancer and neurodegenerative diseases. Its dysregulation links to the progression of cancers where it interacts with the retinoblastoma protein influencing cellular proliferation pathways. In neurodegenerative diseases Prohibitin participates in mechanisms that may affect the development of conditions like Alzheimer's disease associated with its role in mitochondrial dysfunction and oxidative stress. These interactions highlight Prohibitin's importance in both health and disease contexts and its potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed : 11302691, PubMed : 20959514, PubMed : 28017329, PubMed : 31522117). Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity).. In the mitochondria, together with PHB2, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as a chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner (By similarity). Regulates mitochondrial respiration activity playing a role in cellular aging (PubMed : 11302691). The prohibitin complex plays a role of mitophagy receptor involved in targeting mitochondria for autophagic degradation (PubMed : 28017329). Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6 (PubMed : 31522117).. In the nucleus, acts as a transcription coregulator, enhances promoter binding by TP53, a transcription factor it activates, but reduces the promoter binding by E2F1, a transcription factor it represses (PubMed : 14500729). Interacts with STAT3 to affect IL17 secretion in T-helper Th17 cells (PubMed : 31899195).. In the plasma membrane, cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates (By similarity). Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).

See full target information PHB1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 13:4632-4643 PubMed34150043

2021

The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xichang Liu,Haitao Wang,Jiawei Bei,Jun Zhao,Ge Jiang,Xiuhong Liu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29:286-99 PubMed25392270

2014

Cellular origins of cold-induced brown adipocytes in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Hee Lee,Anelia P Petkova,Anish A Konkar,James G Granneman

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prohibitin 抗体

Anti-Prohibitin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (AB154589)

Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (AB154589)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (AB154589)

Western blot - Anti-Prohibitin antibody (AB154589)

Western blot - Anti-Prohibitin antibody (AB154589)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise