Anti-AKAP13/p47 抗体 (ab99377)
Key features and details
- Rabbit polyclonal to AKAP13/p47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AKAP13/p47 antibody
AKAP13/p47 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKAP13/p47 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human AKAP13/p47 aa 2750-2850. NP_009131.2
Database link: Q12802 -
ポジティブ・コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99377の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 308 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 308 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions. -
組織特異性
Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger. -
ドメイン
Both the DH and PH domains are required for transforming activity. -
細胞内局在
Cytoplasm; Cytoplasm. Nucleus and Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 11214 Human
- Omim: 604686 Human
- SwissProt: Q12802 Human
- Unigene: 459211 Human
- Unigene: 710656 Human
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別名
- A kinase (PRKA) anchor protein 13 antibody
- A kinase anchor protein 13 antibody
- A kinase anchoring protein antibody
see all
画像
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All lanes : Anti-AKAP13/p47 antibody (ab99377) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 308 kDa
Exposure time: 10 seconds -
Lane 1 : Detection of Human AKAP13/p47 by Immunoprecipitation from HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded) using 3µg/mg lysate ab99377 lane 2 : Control IgG
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab99377 は 2 報の論文で使用されています。
- Liu Y et al. MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13. Front Oncol 11:754353 (2021). PubMed: 34722307
- Schrade K et al. An AKAP-Lbc-RhoA interaction inhibitor promotes the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells. PLoS One 13:e0191423 (2018). PubMed: 29373579