AB17771

Anti-procollagen type IIA 抗体 [2]

Name: Anti-procollagen type IIA 抗体 [2] (ab17771) | Abcam
Brand: Abcam Limited
SKU: AB17771
Price: 74000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-procollagen type IIA antibody [2]

(2 Reviews)

(4 Publications)

Mouse Monoclonal Collagen II antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.

別名を表示する

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

IHC-P

AbReview9333****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P. The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer. The blocking agent was 1% TSA at 25°C for 1 hour. The primary antibody was incubated (undiluted) at 25°C for 15 hours. A biotinylated anti-mouse was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P. The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

Epitope is located at the amino-terminal part of procollagen type IIA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen type IIA also known as COL2A1 is a precursor of type II collagen important in forming fibrillar collagen networks. This protein has a molecular mass of approximately 146 kDa. Its expression occurs largely in cartilaginous tissues particularly during the early stages of chondrogenesis. Procollagen type IIA is also found in non-skeletal tissues such as the eye indicating its diverse roles during organismal development.

Biological function summary

Procollagen type IIA contributes to the structural integrity of cartilaginous matrices supporting tissue framework and providing tensile strength. Procollagen type IIA is a precursor in the collagen synthesis pathway where it undergoes a series of enzymatic modifications before converting into mature type II collagen fibrils. This protein forms part of a larger collagen synthesis complex and interacts with enzymes like procollagen N-proteinase playing an essential role in matrix formation.

Pathways

Procollagen type IIA participates in the extracellular matrix (ECM) organization pathway. This involvement is key in skeletal development and tissue repair. The protein interacts closely with other structural components like procollagen type IIB to support chondrogenic activities. Also it is part of the transforming growth factor-beta (TGF-beta) signaling pathway which regulates chondrocyte function and differentiation.

Procollagen type IIA mutations are linked to skeletal disorders such as stickler syndrome and achondrogenesis type II. These mutations disrupt normal collagen fiber assembly affecting cartilage function and leading to distinct skeletal abnormalities. In these disorders it is interconnected with proteins like matrix metalloproteinases (MMPs) which influence tissue remodeling and degradation exacerbating the disease conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

See full target information COL2A1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2021:4251763 PubMed34804261

2021

Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qiu,Yumei Zhu,Yafeng Shi,Jingjing Ji,Yingchao Jin

Aging 13:11433-11454 PubMed33839696

2021

Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Jinying Xu,Shuang Lv,Yan Zhao,Dongjie Sun,Yangyang Zheng,Xianglan Li,Lihong Zhang,Guangfan Chi,Yulin Li

Science translational medicine 6:266ra171 PubMed25504882

2014

Protein-releasing polymeric scaffolds induce fibrochondrocytic differentiation of endogenous cells for knee meniscus regeneration in sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Chang H Lee,Scott A Rodeo,Lisa Ann Fortier,Chuanyong Lu,Cevat Erisken,Jeremy J Mao

PloS one 9:e91662 PubMed24626259

2014

Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels.

Applications

IHC-P

Species

Human

Magnus Ø Olderøy,Magnus B Lilledahl,Marianne Sandvold Beckwith,Jan Egil Melvik,Finn Reinholt,Pawel Sikorski,Jan E Brinchmann

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-procollagen type IIA 抗体 [2]

Anti-procollagen type IIA antibody [2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (AB17771)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise