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AB242802

Anti-Procollagen Type I 抗体 [EPR19075-139] - BSA and Azide free (Detector)

Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal COL1A1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

別名を表示する

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

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Sandwich ELISA - Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) (AB242802)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) (AB242802)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab210579).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19075-139

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab242802 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Pro-Collagen I alpha 1. The recommended pair for sandwich ELISA is:
Capture: ab242554, Mouse Pro-Collagen I alpha 1 Capture Antibody (unconjugated)
Detector: ab242802, Mouse Pro-Collagen I alpha 1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen Type 1 also known as Pro-1 collagen is a precursor molecule for collagen Type I which is the most abundant protein in the human body. Its molecular mass is approximately 230 kDa. This protein is synthesized by fibroblasts and is widely expressed in connective tissues including skin tendons and bones. Once synthesized procollagen Type 1 undergoes post-translational modifications and is cleaved by specific enzymes to form mature collagen fibers contributing to the structural integrity of the extracellular matrix.
Biological function summary

The synthesis and processing of procollagen Type 1 play a major role in maintaining tissue strength and elasticity. This molecule is not part of a larger complex but undergoes self-assembly to form the rigid triple-helical structure typical of collagen fibers. These fibers are essential for providing mechanical support to tissues and facilitating cell adhesion. Additionally its presence is often required for normal wound healing processes and tissue regeneration.

Pathways

The secretion and assembly of procollagen Type 1 occur through the secretory pathway. Transforming growth factor-beta (TGF-beta) signaling significantly regulates this process influencing fibrogenesis. This pathway also involves the interaction with proteins such as fibronectin and integrins which are important for aligning collagen fibers within the extracellular matrix. Furthermore procollagen Type 1 engages in the Wnt signaling pathway affecting the arrangement and stability of collagen networks.

Mutations or dysregulation of procollagen Type 1 synthesis may contribute to conditions such as osteogenesis imperfecta and scleroderma. In osteogenesis imperfecta defective collagen leads to brittle bones due to improper fiber formation. In scleroderma an overproduction of collagen causes skin thickening and organ fibrosis. The altered interaction of procollagen Type 1 with proteins like lysyl oxidase can exacerbate these pathological conditions highlighting the significance of proper collagen processing and assembly for tissue health.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information Col1a1
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組合せ製品

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB184993

Anti-Collagen III antibody [EPR17673]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

  • 0
  • 0
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

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