Anti-pro Caspase-12 抗体 (ab8117)
Key features and details
- Rabbit polyclonal to pro Caspase-12
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-pro Caspase-12 antibody
pro Caspase-12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to pro Caspase-12 -
由来種
Rabbit -
特異性
The antibody only recognizes the prodomain, not the activated form. -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse pro Caspase-12 aa 100-200.
Database link: O08736 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8117の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa. |
ターゲット情報
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関連性
Caspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue. -
細胞内局在
Endoplasmic reticulum -
参照データベース
- Entrez Gene: 12364 Mouse
- SwissProt: O08736 Mouse
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別名
- CASP12 antibody
- CASP12P1 antibody
画像
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Western blot - Anti-pro Caspase-12 antibody (ab8117)All lanes : Anti-pro Caspase-12 antibody (ab8117) at 1 µg/ml
Lane 1 : Murine brain tissue lysate with absence of blocking peptide
Lane 2 : Murine brain tissue lysate with presence of blocking peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-12 antibody (ab8117)ab8117 staining Caspase-12 in mouse liver tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-pro Caspase-12 antibody (ab8117)Immunofluorescence of Caspase-12 in Mouse Liver cells using ab8117 at 10 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab8117 は 7 報の論文で使用されています。
- Wang S et al. LncRNA H19 inhibits ER stress induced apoptosis and improves diabetic cardiomyopathy by regulating PI3K/AKT/mTOR axis. Aging (Albany NY) 14:6809-6828 (2022). PubMed: 36044268
- Ji J et al. Activating PPARβ/δ Protects against Endoplasmic Reticulum Stress-Induced Astrocytic Apoptosis via UCP2-Dependent Mitophagy in Depressive Model. Int J Mol Sci 23:N/A (2022). PubMed: 36142731
- Chen W et al. Honokiol inhibits endoplasmic reticulum stress-associated lipopolysaccharide-induced inflammation and apoptosis in bovine endometrial epithelial cells. Exp Ther Med 22:1476 (2021). PubMed: 34765017
- Hu HJ et al. Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress. Front Pharmacol 12:690371 (2021). PubMed: 34950023
- Liu H et al. YAP promotes gastric cancer cell survival and migration/invasion via the ERK/endoplasmic reticulum stress pathway. Oncol Lett 18:6752-6758 (2019). PubMed: 31807184
- Shen B et al. Mechanism of HN-3 cell apoptosis induced by carboplatin: Combination of mitochondrial pathway associated with Ca2+ and the nucleus pathways. Mol Med Rep 18:4978-4986 (2018). PubMed: 30272304
- Fatma N et al. Deficiency of Prdx6 in lens epithelial cells induces ER stress response-mediated impaired homeostasis and apoptosis. Am J Physiol Cell Physiol 301:C954-67 (2011). PubMed: 21677259