Anti-PRMT5 抗体 - C-terminal (ab31751)
Key features and details
- Rabbit polyclonal to PRMT5 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PRMT5 antibody - C-terminal
PRMT5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRMT5 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ab31751 shows weak staining in mouse samples. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). ab31751 shows weak staining in mouse samples. |
ターゲット情報
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機能
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein arginine N-methyltransferase family. -
翻訳後修飾
Disulfide bonds and non-covalent association mediate homooligomers formation. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10419 Human
- Entrez Gene: 27374 Mouse
- Omim: 604045 Human
- SwissProt: O14744 Human
- SwissProt: Q8CIG8 Mouse
- Unigene: 367854 Human
- Unigene: 196585 Mouse
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別名
- 72 kDa ICln binding protein antibody
- 72 kDa ICln-binding protein antibody
- ANM5_HUMAN antibody
see all
画像
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All lanes : Anti-PRMT5 antibody - C-terminal (ab31751) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab31751 は 18 報の論文で使用されています。
- Erazo T et al. TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphoma. Nat Commun 13:5676 (2022). PubMed: 36167829
- Lewis BW et al. PRMT5 in T Cells Drives Th17 Responses, Mixed Granulocytic Inflammation, and Severe Allergic Airway Inflammation. J Immunol 208:1525-1533 (2022). PubMed: 35288471
- Amici SA et al. PRMT5 Promotes Cyclin E1 and Cell Cycle Progression in CD4 Th1 Cells and Correlates With EAE Severity. Front Immunol 12:695947 (2021). PubMed: 34168658
- McKinney DC et al. Discovery of a First-in-Class Inhibitor of the PRMT5-Substrate Adaptor Interaction. J Med Chem 64:11148-11168 (2021). PubMed: 34342224
- Webb LM et al. Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity. J Clin Invest 130:1683-1698 (2020). PubMed: 32091410