Anti-PRMT1 抗体 [MAT-B12] (ab7027)
Key features and details
- Mouse monoclonal [MAT-B12] to PRMT1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-PRMT1 antibody [MAT-B12]
PRMT1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MAT-B12] to PRMT1 -
由来種
Mouse -
特異性
This Mab reacts with human arginine N-Methyl Transferase -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Fusion protein corresponding to Human PRMT1.
Database link: Q99873 -
ポジティブ・コントロール
- Tested on HELA whole cell extract and transfected Cos cells (recombinant protein PRMT1). The results showed good reactivity of the antibody in both cases. ICC/IF - MCF-7 cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MAT-B12 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the protein arginine N-methyltransferase family. -
細胞内局在
Nucleus. Cytoplasm > cytosol. - Information by UniProt
-
参照データベース
- Entrez Gene: 3276 Human
- Omim: 602950 Human
- SwissProt: Q99873 Human
- Unigene: 20521 Human
-
別名
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
see all
画像
-
Immunofluorecent analysis of MCF-7 cells labelling PRMT1 with ab7027
-
Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (18)
ab7027 は 18 報の論文で使用されています。
- Sung BY et al. Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1. J Clin Invest 132:N/A (2022). PubMed: 35040433
- Wu D et al. CTNNAL1 participates in the regulation of mucus overproduction in HDM-induced asthma mouse model through the YAP-ROCK2 pathway. J Cell Mol Med 26:1656-1671 (2022). PubMed: 35092120
- Hsu JH et al. PRMT1-Mediated Translation Regulation Is a Crucial Vulnerability of Cancer. Cancer Res 77:4613-4625 (2017). PubMed: 28655788
- Wang YC et al. Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8. PLoS One 12:e0185042 (2017). PubMed: 28934323
- Esse R et al. Protein arginine methylation is more prone to inhibition by S-adenosylhomocysteine than DNA methylation in vascular endothelial cells. PLoS One 8:e55483 (2013). WB ; Human . PubMed: 23408989