JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109389

Anti-PRKX 抗体 [EPR4840]

Anti-PRKX antibody [EPR4840]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal PRKX antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

PKX1, PRKX, cAMP-dependent protein kinase catalytic subunit PRKX, PrKX, Protein kinase X, Protein kinase X-linked, Serine/threonine-protein kinase PRKX, Protein kinase PKX1

1 Images
Western blot - Anti-PRKX antibody [EPR4840] (AB109389)
  • WB

Unknown

Western blot - Anti-PRKX antibody [EPR4840] (AB109389)

All lanes:

Western blot - Anti-PRKX antibody [EPR4840] (ab109389) at 1/1000 dilution

Lane 1:

A549 cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Lane 4:

Human fetal kidney lysates at 10 µg

Lane 5:

Human fetal brain lysates at 10 µg

Predicted band size: 41 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-PRKX antibody [EPR4840] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4840

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PRKX also known as Protein Kinase X is a serine/threonine-protein kinase with a molecular mass of about 37 kDa. This protein is part of the cAMP-dependent protein kinase family. Scientists observe PRKX expression mainly in the kidney heart and central nervous system. It helps regulate multiple functions by phosphorylating various substrates which are important to cell signaling and communication. The presence of the protein in specific tissues suggests a significant role in development and organ function.
Biological function summary

The protein functions as an important regulator in cellular signaling processes. PRKX interacts with other proteins as a monomer rather than as part of a larger complex. Its activity influences cellular growth differentiation and gene expression. The kinase activity shows high involvement in developmental pathways across numerous cell types. Additionally its interactions affect cellular features like polarization and motility which are important for normal physiological processes.

Pathways

PRKX serves important roles in the PKA signaling pathway and the renal development pathway. PKA signaling involves cAMP as a second messenger and PRKX acts downstream as a phosphorylating agent for various targets within the cell. In the renal development pathway PRKX collaborates with other protein kinases like PRKY which shares a similar sequence and function reflecting redundancy and specialization in developmental roles in kidneys.

The role of PRKX becomes significant in congenital kidney disorders particularly certain types of cystic kidney diseases. The protein's influence on renal development highlights its connection to these conditions. Additionally anomalies in PRKX expression or function can link to central nervous system disorders influencing neurological development and function. Within these contexts PRKX often interacts with related protein networks involving kinases and substrates important for maintaining cellular health and functionality.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
See full target information PRKX
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 41:43-56 PubMed30365128

2018

PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tang,Wenjin Liang,Yong Liao,Zeming Li,Yan Wang,Chao Yan

The Journal of biological chemistry 289:24226-37 PubMed25028512

2014

Transforming growth factor (TGF)-β-activated kinase 1 (TAK1) activation requires phosphorylation of serine 412 by protein kinase A catalytic subunit α (PKACα) and X-linked protein kinase (PRKX).

Applications

WB

Species

Unspecified reactive species

Chuan Ouyang,Li Nie,Meidi Gu,Ailing Wu,Xu Han,Xiaojian Wang,Jianzhong Shao,Zongping Xia
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB154739

Anti-PRKX antibody

primary-antibodies

prkx-antibody-ab154739

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com