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リコンビナントRabMAb

Anti-PRKAR2B 抗体 [EP2649Y] (ab75993)

Submit a review Submit a question References (20)
Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
  • Anti-PRKAR2B antibody [EP2649Y] (ab75993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2649Y] to PRKAR2B
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PRKAR2B antibody [EP2649Y]
    PRKAR2B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2649Y] to PRKAR2B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human PRKAR2B (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • IHC-P: Rat, Mouse, and Human fat tissue sections. IP: Human brain lysate. ICC/IF: Neuro-2a cells. WB: Human, mouse and rat brain lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP2649Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75993の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/25000 - 1/50000.
IHC-P
1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary.
IP
1/30.
ICC/IF
1/50.
特記事項
WB
1/1000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/25000 - 1/50000.
IHC-P
1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary.
IP
1/30.
ICC/IF
1/50.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

  • 組織特異性

    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

  • 配列類似性

    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.

  • 翻訳後修飾

    Phosphorylated by the activated catalytic chain.

  • 細胞内局在

    Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI451071 antibody
    • AW061005 antibody
    • cAMP dependent protein kinase type II beta regulatory chain antibody
    • cAMP dependent protein kinase type II beta regulatory subunit antibody
    • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
    • H RG363E19.2 antibody
    • KAP3_HUMAN antibody
    • MGC116401 antibody
    • Pkarb2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2B antibody
    • Protein kinase cAMP dependent regulatory type II beta antibody
    • RATDNA antibody
    • RII beta antibody
    • RII(beta) antibody
    • RIIbeta antibody
    • WUGSC:H RG363E19.2 antibody
    see all

画像

  • Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Lanes 1-2 : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution (Purified)
    Lane 3 : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution

    Lane 1 : Human brain lysate at 15 µg
    Lane 2 : Mouse brain lysate at 20 µg
    Lane 3 : Rat brain lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)

    Purified ab75993 at 1/30 dilution (2µg) immunoprecipitating PRKAR2B in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab75993 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunocytochemistry/ Immunofluorescence - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling PRKAR2B with purified ab75993 at 1/50 dilution (8.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Anti-PRKAR2B antibody [EP2649Y] (ab75993)

参考文献 (20)

ab75993 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75993 は 20 報の論文で使用されています。

  • Roa JN  et al. Protein Kinase A in Human Retina: Differential Localization of Cβ, Cα, RIIα, and RIIβ in Photoreceptors Highlights Non-redundancy of Protein Kinase A Subunits. Front Mol Neurosci 14:782041 (2021). PubMed: 34867193
  • Zeng XJ  et al. A2A R inhibition in alleviating spatial recognition memory impairment after TBI is associated with improvement in autophagic flux in RSC. J Cell Mol Med 24:7000-7014 (2020). PubMed: 32394486
  • Lin CX  et al. Nobiletin inhibits cell growth through restraining aerobic glycolysis via PKA-CREB pathway in oral squamous cell carcinoma. Food Sci Nutr 8:3515-3524 (2020). PubMed: 32724614
  • Zheng F  et al. P2Y12 deficiency in mouse impairs noradrenergic system in brain, and alters anxiety-like neurobehavior and memory. Genes Brain Behav 18:e12458 (2019). PubMed: 29341465
  • Zhang F  et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). PubMed: 30651859
View all Publications for this product

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