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RabMAb

Anti-PRKAR2B 抗体 [EP2648] (ab75996)

Submit a review Submit a question References (4)
Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996)
  • Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (ab75996)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (ab75996)

Key features and details

  • Rabbit monoclonal [EP2648] to PRKAR2B
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PRKAR2B antibody [EP2648]
    PRKAR2B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2648] to PRKAR2B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Fetal brain, human testis and NIH/3T3 cell lysates. IHC-P: Human brain tissue. Flow Cyt: SHSY-5Y cells

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP2648

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75996の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

  • 組織特異性

    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

  • 配列類似性

    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.

  • 翻訳後修飾

    Phosphorylated by the activated catalytic chain.

  • 細胞内局在

    Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI451071 antibody
    • AW061005 antibody
    • cAMP dependent protein kinase type II beta regulatory chain antibody
    • cAMP dependent protein kinase type II beta regulatory subunit antibody
    • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
    • H RG363E19.2 antibody
    • KAP3_HUMAN antibody
    • MGC116401 antibody
    • Pkarb2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2B antibody
    • Protein kinase cAMP dependent regulatory type II beta antibody
    • RATDNA antibody
    • RII beta antibody
    • RII(beta) antibody
    • RIIbeta antibody
    • WUGSC:H RG363E19.2 antibody
    see all

画像

  • Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996)
    All lanes : Anti-PRKAR2B antibody [EP2648] (ab75996) at 1/20000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Human testis lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (ab75996)

    Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (ab75996)

    ab75996, at 1/100 dilution, staining PRKAR2B in paraffin-embedded human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

参考文献 (4)

ab75996 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75996 は 4 報の論文で使用されています。

  • Yin C  et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). PubMed: 30990811
  • Cao S  et al. Intrathecal TRPM8 blocking attenuates cold hyperalgesia via PKC and NF-?B signaling in the dorsal root ganglion of rats with neuropathic pain. J Pain Res 12:1287-1296 (2019). PubMed: 31114308
  • Anastácio A  et al. Protein profile of mouse ovarian follicles grown in vitro. Mol Hum Reprod 23:827-841 (2017). PubMed: 29069483
  • Corredor RG  et al. Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth. J Neurosci 32:7734-44 (2012). IHC-Fr ; Rat . PubMed: 22649251

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