Anti-PRKAR1B 抗体 (ab167325)
Key features and details
- Mouse polyclonal to PRKAR1B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PRKAR1B antibody -
製品の詳細
Mouse polyclonal to PRKAR1B -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PRKAR1B aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_002726.1 -
ポジティブ･コントロール
- PRKAR1B transfected 293T cell lysate; HeLa cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
組織特異性
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. -
配列類似性
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains. -
翻訳後修飾
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity. - Information by UniProt
-
参照データベース
- Entrez Gene: 5575 Human
- Omim: 176911 Human
- SwissProt: P31321 Human
- Unigene: 520851 Human
-
別名
- cAMP dependent protein kinase type I beta regulatory subunit antibody
- cAMP-dependent protein kinase type I-beta regulatory subunit antibody
- KAP1_HUMAN antibody
see all
画像
-
Western blot - Anti-PRKAR1B antibody (ab167325)All lanes : Anti-PRKAR1B antibody (ab167325) at 1 µg/ml
Lane 1 : PRKAR1B transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1B antibody (ab167325)Immunofluorescent analysis of PRKAR1B expression in HeLa cells using ab167325 at 10 µg/ml dilution.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab167325 は論文での使用が確認できていません。
