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リコンビナントRabMAb

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) 抗体 [EPR1044(2)] (ab187660)

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Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
  • Flow Cytometry (Intracellular) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1044(2)] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)]
    PRK3+PRK2+PRK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1044(2)] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Lysate from Hela cells treated with Okadaic acid + Calyculin A; Human astrocytoma and colon tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187660の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 99, 112, 104 kDa.
特記事項
Flow Cyt (Intra)
1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 99, 112, 104 kDa.

ターゲット情報

画像

  • Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with Okadaic acid + Calyculin A
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 99, 112, 104 kDa



    Based on the sequence analysis, ab187660 recognizes three proteins with the predicted MWs of 99KDa, 112KDa and 104KDa, respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Flow Cytometry (Intracellular) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Intracellular flow cytometric analysis ofpermeabilized HeLa cells, untreated (green) or Okadaic acid + Calyculin A-treated (red) using ab187660 at 1/280 dilution, and Okadaic acid + Calyculin A-treated HeLa cells using ab187660preincubated with phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (blue) or non-phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (orange). Cells were fixed with 2% paraformaldehyde. Secondary antibody was aGoat anti IgG (FITC) at 1/150 dilution. 

     

     

  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

参考文献 (0)

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