AB2777

Anti-Prion protein PrP 抗体 [F89/160.1.5]

Name: Anti-Prion protein PrP 抗体 [F89/160.1.5] (ab2777) | Abcam
Brand: Abcam Limited
SKU: AB2777
Price: 73000 JPY
Availability: InStock

Anti-Prion protein PrP antibody [F89/160.1.5]

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(2 Publications)

Mouse Monoclonal Prion protein PrP antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Cow PRNP aa 100-200.

別名を表示する

CD230, PRP, PRNP, Major prion protein, PrP, Major scrapie-associated fibril protein 1

1 Images

Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (AB2777)

Flow Cyt

Unknown

Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (AB2777)

Overlay histogram showing SH-SH5Y cells stained with ab2777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2777, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

F89/160.1.5

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Synthetic Peptide within Cow PRNP aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P10279

エピトープ

This antibody is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg/cells for 6 µg/cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

Preservative: 0.05% Sodium azide Constituents: 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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ターゲットの情報

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

See full target information PRNP

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental biology and medicine (Maywood, N.J.) 236:466-76 PubMed21444369

2011

Selection and characterization of DNA aptamers against PrP(Sc).

Applications

Species

Unspecified reactive species

Ping Wang,Kristen L Hatcher,Jason C Bartz,Shu G Chen,Pamela Skinner,Juergen Richt,Hong Liu,Srinand Sreevatsan

Journal of clinical microbiology 36:1750-5 PubMed9620413

1998

Monoclonal antibody F89/160.1.5 defines a conserved epitope on the ruminant prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

K I O'Rourke,T V Baszler,J M Miller,T R Spraker,I Sadler-Riggleman,D P Knowles

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Prion protein PrP 抗体 [F89/160.1.5]

Anti-Prion protein PrP antibody [F89/160.1.5]

Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (AB2777)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

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Primary Antibodies

Primary Antibodies

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組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise