Anti-PRG2 抗体 (ab187523)
Key features and details
- Rabbit polyclonal to PRG2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRG2 antibody
PRG2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRG2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human Major Basic Protein aa 1 to the C-terminus.
Database link: P13727 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. -
組織特異性
High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon. -
配列類似性
Contains 1 C-type lectin domain. -
発生段階
Levels of the proform increase in serum and placenta during pregnancy. -
翻訳後修飾
Nitrated. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5553 Human
- Entrez Gene: 19074 Mouse
- Omim: 605601 Human
- SwissProt: P13727 Human
- SwissProt: Q61878 Mouse
- Unigene: 512633 Human
- Unigene: 142727 Mouse
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別名
- BMPG antibody
- EMBP antibody
- Eosinophil granule major basic protein antibody
see all
画像
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Western blot - Anti-PRG2 antibody (ab187523)All lanes : Anti-PRG2 antibody (ab187523) at 1/1000 dilution
Lane 1 : L-02 (human adenocarcinoma cell line) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 5 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse spleen tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer: 3% NFDM/TBST
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab187523 は 5 報の論文で使用されています。
- Chetty A et al. Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection. Cell Host Microbe 29:579-593.e5 (2021). PubMed: 33857419
- Liang C et al. Construction of an irreversible allergic rhinitis-induced olfactory loss mouse model. Biochem Biophys Res Commun 513:635-641 (2019). PubMed: 30981508
- Jin H et al. Activated Eosinophils are Present in Esophageal Muscle in Patients with Achalasia of the Esophagus. Med Sci Monit 24:2377-2383 (2018). PubMed: 29672471
- Engmann J et al. Increased Activity and Apoptosis of Eosinophils in Blister Fluids, Skin and Peripheral Blood of Patients with Bullous Pemphigoid. Acta Derm Venereol 97:464-471 (2017). PubMed: 27868136
- Tamarozzi F et al. Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis. Sci Rep 6:35559 (2016). IHC-P ; Human . PubMed: 27752109