AB264536

Anti-PREX1 抗体 [6F12] - BSA and Azide free

Anti-PREX1 antibody [6F12] - BSA and Azide free

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal PREX1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

別名を表示する

KIAA1415, PREX1, P-Rex1

2 Images

Lab

Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (AB264536)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : MDA-MB-231 (PMID : 21172654).

Exposure time : 59 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264535).

All lanes:

Western blot - Anti-PREX1 antibody [6F12] (<a href='/products/primary-antibodies/prex1-antibody-6f12-ab264535'>ab264535</a>) at 1/1000 dilution

Lane 1:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 186 kDa

Observed band size: 186 kDa

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Lab

Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (AB264536)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 180 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264535).

All lanes:

Western blot - Anti-PREX1 antibody [6F12] (<a href='/products/primary-antibodies/prex1-antibody-6f12-ab264535'>ab264535</a>) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 186 kDa

Observed band size: 186 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

6F12

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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製品の詳細

ab264536 is the carrier-free version of ab264535.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PREX1 protein also known as phosphatidylinositol 345-trisphosphate-dependent Rac exchange factor 1 operates as a guanine nucleotide exchange factor (GEF). It facilitates the activation of the small GTPase RAC1 by exchanging GDP for GTP. The protein has a molecular mass of approximately 185 kDa. PREX1 is widely expressed in various tissues with higher levels observed in brain heart and leukocytes. Additionally it contains several distinct domains including the DH domain responsible for its GEF activity PH domain for lipid binding and DEP domain aiding in signaling pathway integration.

Biological function summary

PREX1 plays a fundamental role in modulating cellular processes such as cell migration growth and survival. It interacts with RAC1 enabling the restructuring of the actin cytoskeleton and promoting cell motility. PREX1 does not only function in isolation but is often found within complexes that influence RAC1 activity. It cooperates with proteins like PI3K (phosphoinositide 3-kinase) enhancing downstream signaling events integral to cellular function.

Pathways

PREX1 participates in pathways like PI3K/Akt and RAC1 signaling. These pathways are pivotal in regulating cellular responses to external stimuli. Interaction with PI3K leads to PREX1 activation by PIP3 which in turn activates RAC1. This association is important for signal transduction involved in cellular growth and survival. Furthermore PREX1's interaction with proteins like AKT and mTOR highlights its involvement in metabolic regulation and cellular homeostasis.

PREX1 exhibits significant correlation with cancer and cardiovascular diseases. Aberrant PREX1 expression or function often leads to altered RAC1 signaling contributing to tumorigenesis and metastasis in cancers such as breast cancer. The dysregulation of PREX1 also associates with cardiovascular conditions by influencing vascular tone and heart muscle contractility. These disease associations emphasize interactions with proteins like PI3K and RAC1 highlighting their roles in disease pathophysiology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.

See full target information PREX1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Current biology : CB 15:1867-73 PubMed16243035

2005

P-Rex1 regulates neutrophil function.

Applications

Unspecified application

Species

Unspecified reactive species

Heidi C E Welch,Alison M Condliffe,Laura J Milne,G John Ferguson,Kirsti Hill,Louise M C Webb,Klaus Okkenhaug,W John Coadwell,Simon R Andrews,Marcus Thelen,Gareth E Jones,Phillip T Hawkins,Len R Stephens

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PREX1 抗体 [6F12] - BSA and Azide free

Anti-PREX1 antibody [6F12] - BSA and Azide free

Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (AB264536)

Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (AB264536)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise