Name: Anti-Presenilin 2/AD5 antibody [EP1515Y]
Brand: RabMAb
SKU: ab51249
Rating: 4 (7 reviews)

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Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1515Y] to Presenilin 2/AD5
Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-Presenilin 2/AD5 antibody [EP1515Y]
Presenilin 2/AD5 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP1515Y] to Presenilin 2/AD5
由来種

Rabbit
特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Presenilin 2/AD5 aa 300-400 (C terminal). The exact sequence is proprietary.
ポジティブ･コントロール
- WB: HeLa, HepG2, C2C12, C6, and Neuro-2a lysates; IHC-P: Human cerebrum tissue; ICC/IF: PC-12 cells; Flow Cyt (intra): HeLa cells; IP: HeLa and PC-12 cell lysates.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP1515Y
アイソタイプ

IgG
研究分野
- Neuroscience
- Diseases

Alternative Versions
- Anti-Presenilin 2/AD5 antibody [EP1515Y] - BSA and Azide free (ab239841)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab51249の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/50.
WB	(4)	1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
IHC-P	(1)	1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
IP	(1)	1/20.
ICC/IF		1/50 - 1/250.

特記事項
Flow Cyt (Intra) 1/50.
WB 1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
IP 1/20.
ICC/IF 1/50 - 1/250.

機能

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
組織特異性

Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
関連疾患

Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
配列類似性

Belongs to the peptidase A22A family.
ドメイン

The PAL motif is required for normal active site conformation.
翻訳後修飾

Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
Phosphorylated on serine residues.
細胞内局在

Endoplasmic reticulum membrane. Golgi apparatus membrane.
Target information above from: UniProt accession P49810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5664 Human
- Entrez Gene: 19165 Mouse
- Entrez Gene: 81751 Rat
- Omim: 600759 Human
- SwissProt: P49810 Human
- SwissProt: Q61144 Mouse
- SwissProt: O88777 Rat
- Unigene: 25363 Human
- Unigene: 330850 Mouse
- Unigene: 11045 Rat
see all
別名
- AD3L antibody
- AD3LP antibody
- AD4 antibody
- AD5 antibody
- Alzheimer disease 4 antibody
- CMD1V antibody
- E5-1 antibody
- OTTHUMP00000035671 antibody
- OTTHUMP00000035672 antibody
- OTTHUMP00000228286 antibody
- OTTHUMP00000228288 antibody
- Presenilin 2 (Alzheimer disease 4) antibody
- Presenilin 2 antibody
- Presenilin-2 CTF subunit antibody
- PS-2 antibody
- PS2 antibody
- Psen2 antibody
- PSN2_HUMAN antibody
- PSNL2 antibody
- STM-2 antibody
- STM2 antibody
see all

Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

All lanes : Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249) at 1/20000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Presenilin 2/AD5 with purified ab51249 at 1/500 dilution (0.51 µg/ml). Heat mediated antigen retrieval using Bond^TM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

ab51249 (purified ) at 1/20 dilution (1ug) immunoprecipitating Presenilin 2/AD5 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab51249 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Presenilin was immunoprecipitated from 1 mg of HeLa whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Input: HeLa whole cell extract 10 µg.

IP (+): ab51249 IP in HeLa whole cell extract.

IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Presenilin was immunoprecipitated from 1 mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Input: PC-12 whole cell extract 10 µg.

IP (+): ab51249 IP in PC-12 whole cell extract.

IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab51249 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab51249 は 21 報の論文で使用されています。

Gao C et al. Presenilin2 D439A Mutation Induces Dysfunction of Mitochondrial Fusion/Fission Dynamics and Abnormal Regulation of GTPase Activity. Mol Neurobiol 61:5047-5070 (2024). PubMed: 38159198
Ulku I et al. Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis. Sci Rep 13:2216 (2023). PubMed: 36750595
Depp C et al. Myelin dysfunction drives amyloid-β deposition in models of Alzheimer's disease. Nature 618:349-357 (2023). PubMed: 37258678
Sun Y et al. Apolipoprotein E4 inhibits γ-secretase activity via binding to the γ-secretase complex. J Neurochem 164:858-874 (2023). PubMed: 36582176
Klein M et al. Converging roles of PSENEN/PEN2 and CLN3 in the autophagy-lysosome system. Autophagy 18:2068-2085 (2022). PubMed: 34964690

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-8 of 8 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Dopaminergic Neurons)

Gel Running Conditions

Reduced Denaturing (NuPage 4-12% MES buffer)

Loading amount

30 µg

Specification

Dopaminergic Neurons

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0

Permeabilization

Specification

brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sulieman Minhas

Verified customer

投稿 Dec 13 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))

Gel Running Conditions

Reduced Denaturing (12.5% SDS gel)

Loading amount

10 µg

Specification

human iPS cell derived neurons (healthy/diseased)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 25 2016

Application

Western blot

Sample

Mouse Cell lysate - other (WT AND KO MEF)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

WT AND KO MEF

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 08 2010

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (HELA)

Total protein in input

200 µg

Immuno-precipitation step

Protein G

Specification

HELA

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 22 2010

Application

Immunocytochemistry

Sample

Human Cultured Cells (HeLa)

Permeabilization

Yes - Triton-X-0.5%

Specification

HeLa

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 14 2010

Application

Western blot

Sample

Human Cell lysate - whole cell (HELA)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

HELA

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 30 2010

Question

please send immunogen range

Abcam community

Verified customer

Asked on May 25 2012

Answer

Thank you for contacting us.

The immunogen is located within residues 300 - 350 for this antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on May 25 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (21)

レビューと Q&A

Western blot abreview for Anti-Presenilin 2/AD5 antibody [EP1515Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Presenilin 2/AD5 antibody [EP1515Y]

Western blot abreview for Anti-Presenilin 2/AD5 antibody [EP1515Y]

Western blot abreview for Anti-Presenilin 2/AD5 antibody [EP1515Y]

Immunoprecipitation abreview for Anti-Presenilin 2 / AD5 antibody [EP1515Y]

Immunocytochemistry abreview for Anti-Presenilin 2/AD5 antibody [EP1515Y]

Western blot abreview for Anti-Presenilin 2 / AD5 antibody [EP1515Y]