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Anti-Presenilin 1/PS-1 抗体 [APS 18] (ab15458)

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Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
  • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

Key features and details

  • Mouse monoclonal [APS 18] to Presenilin 1/PS-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Presenilin 1/PS-1 protein (ab114286)
キット
Product image
ECL Substrate Kit (Very High Sensitivity) (ab133408)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Presenilin 1/PS-1 antibody [APS 18]
    Presenilin 1/PS-1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [APS 18] to Presenilin 1/PS-1
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Presenilin 1/PS-1 aa 300-400.
    Database link: P49768

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: T-47D, MCF7, Daudi, SH-SY5Y, Caco-2 cell lysate. IHC-P: Human liver and tonsil tissue. ICC: MCF-7 cells, A2058 melanoma cells, mouse fibroblasts and HeLa cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    APS 18
  • アイソタイプ

    IgG1
  • 研究分野

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Neuroscience
    • Diseases

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Presenilin 1/PS-1 protein (ab114286)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15458の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500. Predicted molecular weight: 53 kDa.
IHC-P
1/20 - 1/200.
ICC/IF
1/50 - 1/200.
特記事項
WB
1/500. Predicted molecular weight: 53 kDa.
IHC-P
1/20 - 1/200.
ICC/IF
1/50 - 1/200.

ターゲット情報

  • 機能

    Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • 組織特異性

    Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • 関連疾患

    Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • 配列類似性

    Belongs to the peptidase A22A family.
  • ドメイン

    The PAL motif is required for normal active site conformation.
  • 翻訳後修飾

    Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • 細胞内局在

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Target information above from: UniProt accession P49768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5663 Human
    • Entrez Gene: 19164 Mouse
    • Omim: 104311 Human
    • SwissProt: P49768 Human
    • SwissProt: P49769 Mouse
    • Unigene: 3260 Human
    • Unigene: 998 Mouse
    • 別名

      • AD3 antibody
      • Ad3h antibody
      • FAD antibody
      • Homo Sapiens Clone CC44 Senilin 1 antibody
      • Presenilin-1 CTF12 antibody
      • Protein S182 antibody
      • PS 1 antibody
      • PS-1 antibody
      • PS1-CTF12 antibody
      • PSEN1 antibody
      • PSN1_HUMAN antibody
      • PSNL1 antibody
      • S182 antibody
      see all

    画像

    • Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      All lanes : Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458) at 1/500 dilution

      Lane 1 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
      Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
      Lane 5 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

      Predicted band size: 53 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in MCF-7 cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in A2058 melanoma cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in HeLa cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)
      Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

      IF showing PS1 in mouse fibroblasts using ab15458.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (3)

    ab15458 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab15458 は 3 報の論文で使用されています。

    • Li B  et al. ADAM10 mediates ectopic proximal tubule development and renal fibrosis through Notch signalling. J Pathol 252:274-289 (2020). PubMed: 32715474
    • Mirzaei M  et al. Upregulation of Proteolytic Pathways and Altered Protein Biosynthesis Underlie Retinal Pathology in a Mouse Model of Alzheimer's Disease. Mol Neurobiol N/A:N/A (2019). PubMed: 30707393
    • Zoltowska KM  et al. Novel interaction between Alzheimer's disease-related protein presenilin 1 and glutamate transporter 1. Sci Rep 8:8718 (2018). PubMed: 29880815

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Do you have a substitution for antibody ab15458, against the same domain of presenilin?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2007

    Answer

    Thank you for your enquiry. The following antibodies were raised against the C-terminal portion of presenilin, as was ab15458. All the immunogens are within the CTF subunit and all are cytoplasmic: ab38323 ab24748 ab16244 ab12272 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jul 31 2007

    Question

    Thank you for your quick reply. We would like to test one more of your antibody as a replacement for ab15456, and found that ab15458 could be a good replacement. We also looked for a N-terminal PS1 antibody but did not find any that was tested against Hum, rat and mouse. Do you have any? Hope that this will turn out OK now.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2005

    Answer

    Let's see - we have the following PS1 antibodies that have been tested for cross-reactivity with human, mouse, and rat and tested in Western blotting: ab15456 (which you tried), ab15458, ab16244 (a new chicken polyclonal), and ab10281 (tested in human, predicted to cross-react with mouse and rat due to sequence homology). So just let me know which you would like. Also, what was the Abcam order number or purchase order number that was used for your order for ab15456? Thank you!

    Read More

    Abcam Scientific Support

    Answered on Feb 07 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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