Anti-PREP1 抗体 (ab154587)
Key features and details
- Rabbit polyclonal to PREP1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PREP1 antibody -
製品の詳細
Rabbit polyclonal to PREP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human PREP1 aa 201-436.
Database link: P55347 -
ポジティブ･コントロール
- WB: HepG2 nuclear lysate; IP: HeLa cell lysate; ICC/IF: HeLa cell lysate;
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特記事項
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154587の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
|
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| ICC/IF |
1/100 - 1/1000.
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| IP |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/1000. |
ターゲット情報
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組織特異性
Ubiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow. -
配列類似性
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 533382 Cow
- Entrez Gene: 5316 Human
- Entrez Gene: 18771 Mouse
- Entrez Gene: 294322 Rat
- Omim: 602100 Human
- SwissProt: Q2HJ84 Cow
- SwissProt: P55347 Human
- SwissProt: O70477 Mouse
see all -
別名
- Homeobox protein PKNOX1 antibody
- Homeobox protein PREP-1 antibody
- Human homeobox containing protein antibody
see all
画像
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Western blot - Anti-PREP1 antibody (ab154587)All lanes : Anti-PREP1 antibody (ab154587) at 1/2000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PREP1 antibody (ab154587)HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. They were stained using ab154587 diluted at 1/1000 (green).
Nuclear staining Hoechst 33342 staining (blue). -
Immunoprecipitation - Anti-PREP1 antibody (ab154587)HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of PREP1 protein by 2 μg of ab154587Western Blot carried out by ab154587 at a 1/1000 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154587 は論文での使用が確認できていません。