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リコンビナントRabMAb

Anti-PREB 抗体 [EPR13252] (ab181212)

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Western blot - Anti-PREB antibody [EPR13252] (ab181212)
  • Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)
  • Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
  • Anti-PREB antibody [EPR13252] (ab181212)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13252] to PREB
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PREB antibody [EPR13252]
    PREB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13252] to PREB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HT-1376 and Raji cell lysates; Human colon and Human endometrial adenocarcinoma tissues; HT-1376 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13252

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181212の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.

ターゲット情報

  • 機能

    Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Contains 3 WD repeats.

  • 細胞内局在

    Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Mammalian guanine nucleotide exchange factor mSec12 antibody
      • Preb antibody
      • PREB_HUMAN antibody
      • Prolactin regulatory binding element protein antibody
      • Prolactin regulatory element binding antibody
      • Prolactin regulatory element binding protein antibody
      • Prolactin regulatory element-binding protein antibody
      • SEC12 antibody
      see all

    画像

    • Western blot - Anti-PREB antibody [EPR13252] (ab181212)
      Western blot - Anti-PREB antibody [EPR13252] (ab181212)
      All lanes : Anti-PREB antibody [EPR13252] (ab181212) at 1/5000 dilution

      Lane 1 : HT-1376 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 45 kDa

    • Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)
      Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)

      Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)
      Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)

      Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

      Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-PREB antibody [EPR13252] (ab181212)
      Anti-PREB antibody [EPR13252] (ab181212)

    参考文献 (0)

    ab181212 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181212 は論文での使用が確認できていません。

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