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AB215202

Anti-Prealbumin 抗体 [EPR20971]

Anti-Prealbumin antibody [EPR20971]

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(5 Publications)

Rabbit Recombinant Monoclonal Prealbumin antibody. Suitable for IP, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 5 publications.

別名を表示する

Transthyretin, Prealbumin, Ttr

5 Images
Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (choroid plexus) tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the endothelial cells of choroid plexus, negative staining in the endothelial cells of blood vessels on mouse brain tissue section (PMID : 19539719; 20462362).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in choroid plexuses (arrows) of mouse cerebrum (PMID : 25758461; PMID : 19032346). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Prealbumin with ab215202 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the hepatic cells of mouse liver tissue section (PMID : 25519307).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse liver (PMID : 25519307; PMID : 19032346). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (AB215202)
  • IP

Supplier Data

Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (AB215202)

Prealbumin was immunoprecipitated from 0.35 mg of mouse plasma with ab215202 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215202 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution

Lane 1 : Mouse plasma 10 μg (Input).

Lane 2 : ab215202 IP in mouse plasma.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215202 in mouse plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

The band shown at 30 kDa is likely to be a dimer of Prealbumin based on the literature (PMID : 19279259).

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (ab215202)

Predicted band size: 15 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Prealbumin antibody [EPR20971] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20971

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

IHC-Fr, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

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Primary Antibodies

AB199074

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prealbumin antibody [EP2929Y] (AB199074)

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Primary Antibodies

AB92469

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR3219] (AB92469)

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Primary Antibodies

AB75815

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

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Primary Antibodies

AB239915

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR3219] - BSA and Azide free (AB239915)

  • 0
  • 0
Primary Antibodies

AB185127

Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

  • 0
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Primary Antibodies

AB133284

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)

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組合せ製品

Assay Kits

AB282297

Mouse Prealbumin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Prealbumin ELISA Kit (AB282297)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

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Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

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Primary Antibodies

AB281588

Anti-MAP2 antibody [RM1010] - Neuronal Marker

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Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p><p>We recommend to optimize primary antibody dilution depending on the tissue being tested.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information Ttr
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

TH open : companion journal to thrombosis and haemostasis 9:a27014242 PubMed41059381

2025

Effects of DOACs on Mouse Melanoma Metastasis and the Inhibitory Mechanism of Edoxaban, a Factor Xa-Specific DOAC.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Hiramoto,Taisei Watanabe,Masashi Imai,Koji Suzuki

Fluids and barriers of the CNS 21:66 PubMed39152442

2024

The hypervirulent Group B Streptococcus HvgA adhesin promotes central nervous system invasion through transcellular crossing of the choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Aznar,Nathalie Strazielle,Lionel Costa,Claire Poyart,Asmaa Tazi,Jean-François Ghersi-Egea,Julie Guignot

Nature 623:772-781 PubMed37968388

2023

Single-cell, whole-embryo phenotyping of mammalian developmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Xingfan Huang,Jana Henck,Chengxiang Qiu,Varun K A Sreenivasan,Saranya Balachandran,Oana V Amarie,Martin Hrabě de Angelis,Rose Yinghan Behncke,Wing-Lee Chan,Alexandra Despang,Diane E Dickel,Madeleine Duran,Annette Feuchtinger,Helmut Fuchs,Valerie Gailus-Durner,Natja Haag,Rene Hägerling,Nils Hansmeier,Friederike Hennig,Cooper Marshall,Sudha Rajderkar,Alessa Ringel,Michael Robson,Lauren M Saunders,Patricia da Silva-Buttkus,Nadine Spielmann,Sanjay R Srivatsan,Sascha Ulferts,Lars Wittler,Yiwen Zhu,Vera M Kalscheuer,Daniel M Ibrahim,Ingo Kurth,Uwe Kornak,Axel Visel,Len A Pennacchio,David R Beier,Cole Trapnell,Junyue Cao,Jay Shendure,Malte Spielmann

International journal of molecular sciences 24: PubMed36835151

2023

Transthyretin Is Commonly Upregulated in the Hippocampus of Two Stress-Induced Depression Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehito Saito-Takatsuji,Yasuo Yoshitomi,Ryo Yamamoto,Takafumi Furuyama,Yasuhito Ishigaki,Nobuo Kato,Hideto Yonekura,Takayuki Ikeda

Scientific reports 9:19689 PubMed31873158

2019

Gender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter Vancamp,Jean-David Gothié,Cristina Luongo,Anthony Sébillot,Karine Le Blay,Lucile Butruille,Maurice Pagnin,Samantha J Richardson,Barbara A Demeneix,Sylvie Remaud
View all publications
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