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AB75815

Anti-Prealbumin 抗体 [EP2929Y]

Anti-Prealbumin antibody [EP2929Y]

5

(5 Reviews)

|

(4 Publications)

Anti-Prealbumin antibody [EP2929Y] (ab75815) is a rabbit monoclonal antibody detecting Prealbumin in Western Blot, Flow Cytometry (Intra), IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2009

別名を表示する

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] (AB75815)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Intracellular Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (AB75815)
  • IP

Lab

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Purified ab75815 at 1/50 dilution (2μg) immunoprecipitating Prealbumin in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab75815 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 16 kDa

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (ab75815)

Predicted band size: 15 kDa

false

Western blot - Anti-Prealbumin antibody [EP2929Y] (AB75815)
  • WB

Unknown

Western blot - Anti-Prealbumin antibody [EP2929Y] (AB75815)

All lanes:

Western blot - Anti-Prealbumin antibody [EP2929Y] (ab75815) at 1/5000 dilution

Lane 1:

human heart lysate at 10 µg

Lane 2:

human placenta lysate at 10 µg

Lane 3:

human brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 16 kDa

false

関連する標識済み抗体及び組成の異なる製品 (10)

  • Carrier free

    Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free

  • 660 APC

    APC Anti-Prealbumin antibody [EP2929Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Prealbumin antibody [EP2929Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Prealbumin antibody [EP2929Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Prealbumin antibody [EP2929Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Prealbumin antibody [EP2929Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Prealbumin antibody [EP2929Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Prealbumin antibody [EP2929Y]

  • HRP

    HRP Anti-Prealbumin antibody [EP2929Y]

  • 578 PE

    PE Anti-Prealbumin antibody [EP2929Y]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2929Y

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB226962

Anti-Prealbumin antibody

Western blot - Anti-Prealbumin antibody (AB226962)

  • 0
  • 0
Primary Antibodies

AB268123

Anti-Prealbumin antibody [CPTC-TTR-1]

Protein Array - Anti-Prealbumin antibody [CPTC-TTR-1] (AB268123)

  • 0
  • 0
Primary Antibodies

AB204997

Anti-Prealbumin antibody [2E10C5]

Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [2E10C5] (AB204997)

  • 5
  • 1
Primary Antibodies

AB188030

Anti-Prealbumin antibody [CL0290]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [CL0290] (AB188030)

  • 0
  • 0
Primary Antibodies

AB215202

Anti-Prealbumin antibody [EPR20971]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

  • 0
  • 0
Primary Antibodies

AB92469

Anti-Prealbumin antibody [EPR3219]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR3219] (AB92469)

  • 5
  • 1
Primary Antibodies

AB239915

Anti-Prealbumin antibody [EPR3219] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR3219] - BSA and Azide free (AB239915)

  • 0
  • 0
Primary Antibodies

AB219391

Anti-Prealbumin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody (AB219391)

  • 0
  • 0
Primary Antibodies

AB106558

Anti-Prealbumin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody (AB106558)

  • 0
  • 0
Primary Antibodies

AB133284

Anti-Prealbumin antibody [EPR2928(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)

  • 0
  • 0
Primary Antibodies

AB230828

Anti-Prealbumin antibody [EPR20971] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] - BSA and Azide free (AB230828)

  • 0
  • 0

組合せ製品

Assay Kits

AB231920

Human Prealbumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

  • 0
  • 0
Assay Kits

AB282297

Mouse Prealbumin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Prealbumin ELISA Kit (AB282297)

  • 0
  • 0
Primary Antibodies

AB52945

Anti-Apolipoprotein A I antibody [EP1368Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)

  • 4
  • 9
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Proteins & Peptides

AB313360

Recombinant TTR Protein (His tag)

Mass Spectrometry - Recombinant TTR Protein (His tag) (AB313360)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/5000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

What is this antibody validated in?
Anti-Prealbumin antibody [EP2929Y] (ab75815) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Prealbumin?
Anti-Prealbumin [EP2929Y] (ab75815) specifically detects a band for Prealbumin (UniProt: P02766) at a molecular weight of 16kDa.

Reviewed by scientists
Anti-Prealbumin [EP2929Y] (ab75815) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP2929Y] also available for your convenience: ab75815, Carrier free - ab185127, Alexa Fluor® 488 - ab199074, PE - ab305865, APC - ab305866, HRP - ab305867, Alkaline Phosphatase - ab308868, Alexa Fluor® 647 - ab310162, Alexa Fluor® 594 - ab310595, Alexa Fluor® 555 - ab312125, Alexa Fluor® 568 - ab312609, Alexa Fluor® 750 - ab321698

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information TTR
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Reproductive biology and endocrinology : RB&E 21:108 PubMed37968664

2023

Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yu,Xinghao Feng,Zhongshan Lin,Xuan Li,Shiyue Su,Huiru Cheng,Yuanyuan Yang,Zhaolian Wei

Nature communications 12:7142 PubMed34880251

2021

Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cheng,Hao-Long Li,Shao-Yan Xi,Xiao-Feng Zhang,Yun Zhu, Le Xing,Yan-Xuan Mo,Mei-Mei Li,Fan-En Kong,Wen-Jie Zhu,Xiao-Gang Chen,Hui-Qing Cui,Zhi-Ming Cao,Yuan-Feng Gong,Yun-Qiang Tang,Yan Zhang,Xin-Yuan Guan,Ning-Fang Ma,Ming Liu

Oncology letters 19:3815-3826 PubMed32391095

2020

Identification of potential cervical cancer serum biomarkers in Thai patients.

Applications

Unspecified application

Species

Unspecified reactive species

Siriporn Keeratichamroen,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Churat Weeraphan,Kittirat Saharat,Narongrit Sritana,Nuttavut Kantathavorn,Kriangpol Wiriyaukaradecha,Thaniya Sricharunrat,N Monique Paricharttanakul,Chirayu Auewarakul,Jisnuson Svasti,Chantragan Srisomsap

eLife 8: PubMed31393263

2019

Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Katsuda,Juntaro Matsuzaki,Tomoko Yamaguchi,Yasuhiro Yamada,Marta Prieto-Vila,Kazunori Hosaka,Atsuko Takeuchi,Yoshimasa Saito,Takahiro Ochiya
View all publications
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Abcam product promise

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