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AB325407

Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture)

Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Peroxiredoxin 3/PRDX3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III

2 Images
Sandwich ELISA - Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture) (AB325407)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture) (AB325407)

Interpolated concentration of PRDX3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : starting concentration of the first point of recovery in 0.025 mg/ml of HAP1 WT and HAP1 PRDX3 KO. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in CVE buffer. We do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture) (AB325407)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PRDX3 antibody [EPR29860-11] to PRDX3 - BSA and Azide free (Capture) (AB325407)

Sandwich ELISA using immunogen PRDX3 protein standard curve with the capture antibody ab325407 dilution at 1 µg/mL and detector antibody ab325408 dilution at 0.0625 µg/mL. The background-subtracted absorbance data are presented as mean ± standard deviation).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29860-11

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This clone is Specific to Human PRDX3 and does not crossreact with PRDX1, PRDX2, and PRDX4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab325407 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PRDX3. The recommended pair for sandwich ELISA is:
Capture: ab325407, Human PRDX3 Capture Antibody (unconjugated)
Detector: ab325408, Human PRDX3 Detector Antibody (unconjugated)
The reference range value is 156.25 pg/ml-5000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed : 17707404, PubMed : 29438714, PubMed : 33889951, PubMed : 7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed : 12492477). Required for the maintenance of physical strength (By similarity).
See full target information PRDX3
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