Anti-PRDM1/Blimp1 抗体 (ab13700)
Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: ICC, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PRDM1/Blimp1 antibody
PRDM1/Blimp1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PRDM1/Blimp1 -
由来種
Goat -
アプリケーション
適用あり: ICC, ChIP, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human PRDM1/Blimp1 aa 800 to the C-terminus (C terminal).
Database link: O75626 -
ポジティブ・コントロール
- ICC: A431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 10 µg/ml.
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ChIP |
Use at an assay dependent concentration. PubMed: 17264218
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC
Use a concentration of 10 µg/ml. |
ChIP
Use at an assay dependent concentration. PubMed: 17264218 |
Flow Cyt
Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
配列類似性
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
翻訳後修飾
Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 538384 Cow
- Entrez Gene: 481947 Dog
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 100154284 Pig
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
see all -
別名
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
画像
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab13700 は 10 報の論文で使用されています。
- Qing K et al. Dysregulated MDR1 by PRDM1/Blimp1 Is Involved in the Doxorubicin Resistance of Non-Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma. Chemotherapy 67:12-23 (2022). PubMed: 34844236
- Li Q et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766
- Okuzaki Y et al. PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Dev Biol 455:32-41 (2019). PubMed: 31271752
- Chinnapaiyan S et al. TGF-ß1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells. Sci Rep 9:12552 (2019). PubMed: 31467373
- Prajapati RS et al. PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants. Development 146:N/A (2019). PubMed: 31806661
- Zhao H et al. GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. Sci Rep 5:7913 (2015). IF . PubMed: 25604641
- Beermann ML et al. Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro. PLoS One 5:e9951 (2010). PubMed: 20376350
- Wang X et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol 29:3832-44 (2009). PubMed: 19433448
- Yan J et al. BLIMP1 regulates cell growth through repression of p53 transcription. Proc Natl Acad Sci U S A 104:1841-6 (2007). ChIP ; Human . PubMed: 17264218
- Parekh S et al. BCL6 programs lymphoma cells for survival and differentiation through distinct biochemical mechanisms. Blood 110:2067-74 (2007). PubMed: 17545502