Anti-PRC1 抗体 [6G2] (ab39069)
![Western blot - Anti-PRC1 antibody [6G2] (ab39069)](https://www.abcam.co.jp/ps/products/39/ab39069/Images/ab39069-52103-anti-prc1-antibody-6g2-western-blot.jpg "Western blot - Anti-PRC1 antibody [6G2] (ab39069)")
Key features and details
- Mouse monoclonal [6G2] to PRC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-PRC1 antibody [6G2]
PRC1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6G2] to PRC1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 1/150 of Human PRC1
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ポジティブ･コントロール
- HeLa cell extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab39069 is an affinity purified IgG. -
ポリ/モノ
モノクローナル -
クローン名
6G2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39069の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. |
ターゲット情報
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機能
Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. -
配列類似性
Belongs to the MAP65/ASE1 family. -
ドメイン
Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain. -
翻訳後修飾
Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. - Information by UniProt
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参照データベース
- Entrez Gene: 9055 Human
- Omim: 603484 Human
- SwissProt: O43663 Human
- Unigene: 366401 Human
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別名
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
see all
画像
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Western blot - Anti-PRC1 antibody [6G2] (ab39069)Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extracts
Secondary
goat anti-mouse
secondary conjugated to HRP
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab39069 は論文での使用が確認できていません。