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AB39069

Anti-PRC1 抗体 [6G2]

Anti-PRC1 antibody [6G2]

4

(1 Review)

|

(1 Publication)

Mouse Monoclonal PRC1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein regulator of cytokinesis 1 aa 1-150.

別名を表示する

Protein regulator of cytokinesis 1, PRC1

1 Images
Western blot - Anti-PRC1 antibody [6G2] (AB39069)
  • WB

Unknown

Western blot - Anti-PRC1 antibody [6G2] (AB39069)

All lanes:

Western blot - Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/mL

All lanes:

Hela cell extracts

Secondary

All lanes:

goat anti-mouse secondary conjugated to HRP

Predicted band size: 72 kDa

Observed band size: 66 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

6G2

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human Protein regulator of cytokinesis 1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O43663

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
ab39069 is an affinity purified IgG.
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PRC1 also known as Protein Regulator of Cytokinesis 1 affiliates with cellular division mechanics. This protein weighs approximately 72 kDa. PRC1 localizes in the nucleus during the interphase and redistributes to the spindle midzone during mitosis. It plays a role in the stabilization of microtubules facilitating proper cytokinesis. Expression of PRC1 occurs in several human tissues notably in dividing cells.
Biological function summary

PRC1 functions in accurately regulating actin and microtubule interaction during cell division. As a part of the centralspindlin complex it ensures correct spindle midzone formation and serves as a marker for the cleavage furrow. Cellular events coordinated by PRC1 involve chromosomal segregation and rapidly proliferating cells express it to maintain integrity during mitotic processes.

Pathways

PRC1 contributes significantly to the mitotic spindle checkpoint. It participates in the Aurora B kinase pathway interacting with proteins like KIF4A and PLK1. This protein ensures the transition from metaphase to anaphase is precise preventing aneuploidy. As a pathway node PRC1 manages signal transduction facilitating cell cycle regulation and fidelity.

Dysregulation of PRC1 expression associates with cancer notably prostate and breast cancer. Tumor cells often display altered PRC1 activity linked to abnormal cell proliferation. The protein interacts with the oncogenic proteins such as p53 indicating a role in tumorigenesis and progression. Exploring therapeutic strategies targeting PRC1 could provide insights into cancer treatment.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed : 17409436).
See full target information Protein regulator of cytokinesis 1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7799 PubMed40921755

2025

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Matsuhashi,Kei K Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S Stewart,Shoji Hata,Daiju Kitagawa
View all publications
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代替製品

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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組合せ製品

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Trial Size

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Primary Antibodies

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Primary Antibodies

AB313703

Anti-TRIP13/PCH2 antibody [EPR27063-80] - BSA and Azide free

RabMAb|KO Validated|Recombinant

Immunoprecipitation - Anti-TRIP13/PCH2 antibody [EPR27063-80] - BSA and Azide free (AB313703)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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