Anti-PRAK/MK5 抗体 (ab93800)
Key features and details
- Rabbit polyclonal to PRAK/MK5
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRAK/MK5 antibody
PRAK/MK5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRAK/MK5 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
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免疫原
Synthetic peptide corresponding to Human PRAK/MK5 aa 423-473.
Database link: NP_620777.1 -
ポジティブ･コントロール
- Whole cell lysate from HeLa and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 54 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Mediates stress-induced small heat shock protein 27 phosphorylation. -
組織特異性
Expressed ubiquitously. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12. -
細胞内局在
Cytoplasm. Nucleus. Also observed in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 535625 Cow
- Entrez Gene: 8550 Human
- Entrez Gene: 100153404 Pig
- Entrez Gene: 713521 Rhesus monkey
- Omim: 606723 Human
- SwissProt: Q8IW41 Human
- Unigene: 413901 Human
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別名
- MAP kinase-activated protein kinase 5 antibody
- MAPK-activated protein kinase 5 antibody
- MAPK5_HUMAN antibody
see all
画像
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Western blot - Anti-PRAK/MK5 antibody (ab93800)All lanes : Anti-PRAK/MK5 antibody (ab93800) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAK/MK5 antibody (ab93800)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK/MK5 with ab93800 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-PRAK/MK5 antibody (ab93800)Detection of PRAK/MK5 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab93800 は論文での使用が確認できていません。