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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PPP2R5E 抗体 [EPR17147] - C-terminal (ab198500)

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Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Flow Cytometry (Intracellular) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Immunoprecipitation - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
  • Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17147] to PPP2R5E - C-terminal
  • Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PPP2R5E antibody [EPR17147] - C-terminal
    PPP2R5E 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17147] to PPP2R5E - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293T and Daudi cell lysates; Human skeletal muscle lysates. IHC: Human cervix carcinoma and bladder transitional cell carcinoma tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cell lysate. IP: HeLa cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab198500の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP
1/75.
ICC/IF
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/2500.
特記事項
WB
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP
1/75.
ICC/IF
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/2500.

ターゲット情報

  • 機能

    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

  • 配列類似性

    Belongs to the phosphatase 2A regulatory subunit B56 family.

  • 翻訳後修飾

    Phosphorylated on serine residues.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2A5E_HUMAN antibody
    • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
    • PP2A B subunit B' epsilon antibody
    • PP2A B subunit B' epsilon isoform antibody
    • PP2A B subunit B56 epsilon antibody
    • PP2A B subunit B56 epsilon isoform antibody
    • PP2A B subunit isoform B''-epsilon antibody
    • PP2A B subunit isoform B'-epsilon antibody
    • PP2A B subunit isoform B56-epsilon antibody
    • PP2A B subunit isoform PR61-epsilon antibody
    • PP2A B subunit isoform R5-epsilon antibody
    • PP2A B subunit PR61 epsilon antibody
    • PP2A B subunit PR61 epsilon isoform antibody
    • PP2A B subunit R5 epsilon antibody
    • PP2A B subunit R5 epsilon isoform antibody
    • PPP2R5E antibody
    • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
    • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
    • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
    see all

画像

  • Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    All lanes : Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP2R5E knockout HeLa cell lysate
    Lane 3 : HEK-293T cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab198500 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab198500 Recombinant Anti-PPP2R5E antibody [EPR17147] - C-terminal was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cervix carcinoma tissue isobserved. Counter-stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast carcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Flow Cytometry (Intracellular) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Flow Cytometry (Intracellular) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PPP2R5E (red) with purified ab198500 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    All lanes : Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500) at 1/5000 dilution

    Lane 1 : HeLa cell lysate at 10 µg
    Lane 2 : 293 cell lysate at 10 µg
    Lane 3 : Human skeletal muscle lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Immunoprecipitation - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    PPP2R5E was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma) whole cell extract with ab198500 at 1/175 dilution. Western blot was performed from the immunoprecipitate using ab198500 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell extract 10µg (Input).

    Lane 2: HeLa whole cell extract

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198500 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue isobserved. Counter-stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)
    Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

参考文献 (0)

ab198500 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab198500 は論文での使用が確認できていません。

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