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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PPP2R5E 抗体 [EPR17146] (ab198290)

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Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (ab198290)
  • Anti-PPP2R5E antibody [EPR17146] (ab198290)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17146] to PPP2R5E
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PPP2R5E antibody [EPR17146]
    PPP2R5E 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17146] to PPP2R5E

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293T, Daudi, C6 and NIH/3T3 cell lysates; Human pancreas and fetal brain tissue lysate; Mouse and rat brain tissue lysates. ICC/IF: C6 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab198290の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF
1/500.
特記事項
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF
1/500.

ターゲット情報

  • 機能

    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

  • 配列類似性

    Belongs to the phosphatase 2A regulatory subunit B56 family.

  • 翻訳後修飾

    Phosphorylated on serine residues.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2A5E_HUMAN antibody
    • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
    • PP2A B subunit B' epsilon antibody
    • PP2A B subunit B' epsilon isoform antibody
    • PP2A B subunit B56 epsilon antibody
    • PP2A B subunit B56 epsilon isoform antibody
    • PP2A B subunit isoform B''-epsilon antibody
    • PP2A B subunit isoform B'-epsilon antibody
    • PP2A B subunit isoform B56-epsilon antibody
    • PP2A B subunit isoform PR61-epsilon antibody
    • PP2A B subunit isoform R5-epsilon antibody
    • PP2A B subunit PR61 epsilon antibody
    • PP2A B subunit PR61 epsilon isoform antibody
    • PP2A B subunit R5 epsilon antibody
    • PP2A B subunit R5 epsilon isoform antibody
    • PPP2R5E antibody
    • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
    • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
    • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
    see all

画像

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP2R5E knockout HeLa cell lysate
    Lane 3 : HEK-293 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor cell) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (ab198290)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor) cells labeling PPP2R5E with ab198290 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on C6 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198290 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290)

参考文献 (0)

ab198290 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab198290 は論文での使用が確認できていません。

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