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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PPP2R1B 抗体 [EPR10159] (ab154833)

Submit a review Q&A (1)
Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
  • Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
  • Anti-PPP2R1B antibody [EPR10159] (ab154833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10159] to PPP2R1B
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PPP2R1B antibody [EPR10159]
    PPP2R1B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10159] to PPP2R1B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human PPP2R1B.
    Database link: P30154

  • ポジティブ･コントロール

    • Caco2 and Jurkat cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10159

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154833の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

  • 配列類似性

    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.

  • ドメイン

    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2AAB_HUMAN antibody
    • beta isoform of regulatory subunit A, protein phosphatase 2 antibody
    • PP2A A beta antibody
    • PP2A subunit A isoform PR65-beta antibody
    • PP2A subunit A isoform R1-beta antibody
    • PP2A, subunit A, PR65 beta isoform antibody
    • PP2A, subunit A, R1 beta isoform antibody
    • PPP2R1B antibody
    • PPP4R1 antibody
    • PR65B antibody
    • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform antibody
    • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform antibody
    • Protein phosphatase 2, regulatory subunit A, beta antibody
    • Protein phosphatase 2, structural/regulatory subunit A, beta antibody
    • protein phosphatase 2, structural/regulatory subunit A, beta isoform antibody
    • serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform antibody
    • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform antibody
    see all

画像

  • Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: Caco2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    All lanes : Anti-PPP2R1B antibody [EPR10159] (ab154833) at 1/1000 dilution

    Lane 1 : Caco2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 66 kDa

  • Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Anti-PPP2R1B antibody [EPR10159] (ab154833)

参考文献 (0)

ab154833 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154833 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Question

Hi
I need to know whether 7288-1 and/or 7301-1 is specific for PP2AA BETA
or whether they also cross react with A alpha. (If you do not know they
answer, and want to send me a sample, I will let you know.)

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Answer

We have not tested 7288-1 or 7301-1 for cross reactivity with PP2AA alpha, however, we have only observed one band in our in-house testing with 7288-1 and we have detected only 2 isoforms of PP2AA beta with 7301-1. It is possible they could react, based on the fact that the immunogen sequences for 7288-1 and 7301-1 have 78% and 75% homology with PP2AA alpha (respectively), but typically homology needs to be at about 85% for there to be good cross-reactivity.

Please let me know if you have any additional questions.

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