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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PPP2R1B 抗体 [EPR10158] (ab154815)

Submit a review Q&A (1)References (3)
Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)
  • Anti-PPP2R1B antibody [EPR10158] (ab154815)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10158] to PPP2R1B
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-PPP2R1B antibody [EPR10158]
    PPP2R1B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10158] to PPP2R1B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human PPP2R1B (internal sequence). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: HEK293T, HeLa, HAP1, Caco 2 and Jurkat whole cell lysate (ab7899). Mouse spleen tissue lysate and Rat spleen tissue lysate, ICC/IF: Caco-2 cells. Flow Cyt (intra): Caco-2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10158

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154815の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

  • 配列類似性

    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.

  • ドメイン

    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2AAB_HUMAN antibody
    • beta isoform of regulatory subunit A, protein phosphatase 2 antibody
    • PP2A A beta antibody
    • PP2A subunit A isoform PR65-beta antibody
    • PP2A subunit A isoform R1-beta antibody
    • PP2A, subunit A, PR65 beta isoform antibody
    • PP2A, subunit A, R1 beta isoform antibody
    • PPP2R1B antibody
    • PPP4R1 antibody
    • PR65B antibody
    • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform antibody
    • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform antibody
    • Protein phosphatase 2, regulatory subunit A, beta antibody
    • Protein phosphatase 2, structural/regulatory subunit A, beta antibody
    • protein phosphatase 2, structural/regulatory subunit A, beta isoform antibody
    • serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform antibody
    • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform antibody
    see all

画像

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PPP2R1B knockout HEK293T cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 66 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab154815 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab154815 Anti-PPP2R1B antibody [EPR10158]  was shown to specifically react with PPP2R1B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266729 (knockout cell lysate ab258133) was used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Intracellular Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma) cells labeling PPP2R1B with unpurified ab154815 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

     

     

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Caco2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab154815 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab154815 was shown to specifically react with PPP2R1B when PPP2R1B knockout samples were used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Immunofluorescence analysis of Caco 2 cells, labeling PPP2R1B using ab154815 at 1/50 dilution.
  • Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Anti-PPP2R1B antibody [EPR10158] (ab154815)

参考文献 (3)

ab154815 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154815 は 3 報の論文で使用されています。

  • Jiang SL  et al. The Downregulation of eIF3a Contributes to Vemurafenib Resistance in Melanoma by Activating ERK via PPP2R1B. Front Pharmacol 12:720619 (2021). PubMed: 34512348
  • Keshri R  et al. PP2A--B55? counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA. J Cell Sci 133:N/A (2020). PubMed: 32591484
  • Lahlali T  et al. Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response. Cell Mol Gastroenterol Hepatol 2:281-301.e9 (2016). PubMed: 28174720

レビューと Q&A

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レビューを送る 質問を送る

Question

Hi
I need to know whether 7288-1 and/or 7301-1 is specific for PP2AA BETA
or whether they also cross react with A alpha. (If you do not know they
answer, and want to send me a sample, I will let you know.)

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Answer

We have not tested 7288-1 or 7301-1 for cross reactivity with PP2AA alpha, however, we have only observed one band in our in-house testing with 7288-1 and we have detected only 2 isoforms of PP2AA beta with 7301-1. It is possible they could react, based on the fact that the immunogen sequences for 7288-1 and 7301-1 have 78% and 75% homology with PP2AA alpha (respectively), but typically homology needs to be at about 85% for there to be good cross-reactivity.

Please let me know if you have any additional questions.

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