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リコンビナント

Anti-PPP2R1A 抗体 [6G3] (ab24736)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
  • Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
  • Anti-PPP2R1A antibody [6G3] (ab24736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6G3] to PPP2R1A
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PPP2R1A antibody [6G3]
    PPP2R1A 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [6G3] to PPP2R1A

  • 由来種

    Rat

  • アプリケーション

    適用あり: ICC/IF, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein corresponding to Human PPP2R1A.
    Database link: P30153

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: HeLa, Jurkat ,Ramos, HepG2, C6 and RAW 264.7 whole cell lysate. ICC/IF: HeLa, RAW 264.7 and C6 cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab24736の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 7.85 µg/ml.
WB
Use a concentration of 0.785 µg/ml. Predicted molecular weight: 65 kDa.
特記事項
ICC/IF
Use a concentration of 7.85 µg/ml.
WB
Use a concentration of 0.785 µg/ml. Predicted molecular weight: 65 kDa.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.

  • 配列類似性

    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.

  • ドメイン

    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

  • 細胞内局在

    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 2AAA_HUMAN antibody
      • 6330556D22Rik antibody
      • Alpha isoform of regulatory subunit A, protein phosphatase antibody
      • DKFZp470L0914 antibody
      • Medium tumor antigen-associated 61 kDa protein antibody
      • MGC128399 antibody
      • MGC786 antibody
      • MGC83000 antibody
      • MGC95083 antibody
      • PP2A Aalpha antibody
      • PP2A antibody
      • PP2A subunit A isoform PR65-alpha antibody
      • PP2A subunit A isoform R1-alpha antibody
      • PP2A, subunit A, PR65-alpha isoform antibody
      • PP2A, subunit A, R1-alpha isoform antibody
      • PP2AAALPHA antibody
      • Ppp2r1a antibody
      • ppp2r1a-b antibody
      • PR65 antibody
      • PR65-alpha antibody
      • PR65A antibody
      • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
      • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
      • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
      • Protein phosphatase 2 regulatory subunit A alpha antibody
      • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
      • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
      • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
      • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
      Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
      -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    • Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)
      Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)
      All lanes : Anti-PPP2R1A antibody [6G3] (ab24736) at 0.785 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
      Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

      Predicted band size: 65 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
      Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
      -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
      Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
      -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    • Anti-PPP2R1A antibody [6G3] (ab24736)
      Anti-PPP2R1A antibody [6G3] (ab24736)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab24736 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab24736 は 1 報の論文で使用されています。

    • Kremmer E  et al. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells. Mol Cell Biol 17:1692-701 (1997). PubMed: 9032296

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