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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PPP2CB 抗体 [EPR11786(B)] (ab168371)

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Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
  • Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
  • Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11786(B)] to PPP2CB
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PPP2CB antibody [EPR11786(B)]
    PPP2CB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11786(B)] to PPP2CB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Human PPP2CB.
    Database link: P62714

  • ポジティブ･コントロール

    • 293T, HeLa, Human fetal brain, Human fetal heart and NIH 3T3 lysates; 293T and NIH3T3 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11786(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab168371の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

  • 配列類似性

    Belongs to the PPP phosphatase family. PP-1 subfamily.

  • 翻訳後修飾

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.

  • 細胞内局在

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • PP2A beta antibody
    • PP2A-beta antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2CB antibody
    • Ppp2cb antibody
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isozyme antibody
    • Protein phosphatase 2, catalytic subunit, beta isoform antibody
    • Protein phosphatase 2A catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
    see all

画像

  • Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: PPP2CB knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)

    ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. Ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution

    Lane 1 : 293T lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa

  • Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

参考文献 (4)

ab168371 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab168371 は 4 報の論文で使用されています。

  • Gao R  et al. Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signaling. FEBS J 289:4518-4535 (2022). PubMed: 35068054
  • You Y  et al. Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells. Aging Cell 21:e13617 (2022). PubMed: 35567427
  • Liang Y  et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ 28:2728-2744 (2021). PubMed: 33934104
  • Sun S  et al. Wnt pathway-related three-mRNA clinical outcome signature in bladder urothelial carcinoma: computational biology and experimental analyses. J Transl Med 19:409 (2021). PubMed: 34579753

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