AB168371

Anti-PPP2CB 抗体 [EPR11786(B)]

Anti-PPP2CB antibody [EPR11786(B)]

RabMAb
Recombinant
KO Validated
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(6 Publications)

Rabbit Recombinant Monoclonal PP2AB antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

別名を表示する

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-beta, PPP2CB

2 Images

Lab

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (AB168371)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : PPP2CB knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : A431 whole cell lysate (20 μg)

ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Unknown

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (AB168371)

All lanes:

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

NIH3T3 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11786(B)

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPP2CB also known as protein phosphatase 2 catalytic subunit beta is an enzyme that plays an important role in cellular functions. It plays a catalytic role in dephosphorylating serine and threonine residues in various proteins. The alternative names used for PPP2CB include PP2Acβ and PP2A(C subunit)β. This protein has a mass of approximately 35 kDa and is expressed in a variety of tissues with significant presence in heart skeletal muscle and brain. Researchers note its expression in both cytoplasmic and nuclear locations within cells.

Biological function summary

The protein phosphatase activity of PPP2CB influences many cellular processes. It is a vital part of the protein phosphatase 2 (PP2A) complex which is a major serine/threonine phosphatase in cells. Through its involvement in the PP2A complex PPP2CB regulates cellular processes like cell growth division and apoptosis. The PP2A complex of which PPP2CB is a component remains essential to signaling pathways contributing to the dynamic behavior of cellular phosphorylation-dephosphorylation cycles.

Pathways

Protein phosphatase 2's function aligns PP2CB with key signaling routes such as the MAPK and Wnt pathways. In the MAPK pathway PP2A complex dephosphorylates specific kinases which controls cellular responses to external stimuli. Within the Wnt pathway the regulatory actions of PP2A maintain the appropriate level of pathway activity. The roles of PPP2CB in these pathways connect it to other related proteins like B56 family regulatory subunits in signal transduction networks.

Disruptions in PPP2CB activity link to several conditions including cancer and neurodegenerative diseases. The dysregulation of PPP2CB in cancer is often associated with altered cell proliferation and survival as PP2A is a tumor suppressor complex. In neurodegenerative diseases particularly Alzheimer's aberrant PPP2CB activity disrupts phosphorylation states of tau protein contributing to disease pathogenesis. PPP2CB interacts with other significant proteins like tau in Alzheimer's highlighting its importance in maintaining cellular and neurological health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (Probable). PP2A can modulate the activity of phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).

See full target information PPP2CB

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101519 PubMed40612667

2025

Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Lei Shi,Zijun Meng,Manlin Zhang,Zhiqi Zhang,Yunzhe Li,Kaiwen Du,Muyao Yang,Lin Qiu,Jing Feng,Yuchen He,Jiayun Liu,Hua Zhang,Hongbin Zhang,Tingyuan Lang,Zhuo Yang

Molecular medicine reports 31: PubMed39540350

2024

Silencing PPAP2C inhibits lung adenocarcinoma migration and invasion via the ERK/JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Wenhui Dang,Ting Jiao,Mengying Zhang,Wei Li

Aging cell 21:e13617 PubMed35567427

2022

Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang You,Samuel W Hersh,Roshanak Aslebagh,Scott A Shaffer,Seiko Ikezu,Jesse Mez,Kathryn L Lunetta,Mark W Logue,Lindsay A Farrer,Tsuneya Ikezu

The FEBS journal 289:4518-4535 PubMed35068054

2022

Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Gao,Xin Li,Huiying Gao,Ke Zhao,Xian Liu,Jinfang Liu,Qi Wang,Yaxin Zhu,Hui Chen,Shensi Xiang,Yiqun Zhan,Ronghua Yin,Miao Yu,Hongmei Ning,Xiaoming Yang,Changyan Li

Journal of translational medicine 19:409 PubMed34579753

2021

Wnt pathway-related three-mRNA clinical outcome signature in bladder urothelial carcinoma: computational biology and experimental analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Siqing Sun,Yutao Wang,Jianfeng Wang,Jianbin Bi

Cell death and differentiation 28:2728-2744 PubMed33934104

2021

PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Xiaoli Sun,Mingjie Wang,Qingmiao Lu,Mengru Gu,Lu Zhou,Qing Hou,Mengzhu Tan,Sudan Wang,Xian Xue,Chunsun Dai

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2CB 抗体 [EPR11786(B)]

Anti-PPP2CB antibody [EPR11786(B)]

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (AB168371)

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (AB168371)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise