Anti-PPM1G 抗体 [EPR13362-31] (ab186423)
![Western blot - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219982-anti-ppm1g-antibody-epr13362-31-western-blot.jpg "Western blot - Anti-PPM1G antibody [EPR13362-31] (ab186423)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13362-31] to PPM1G
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PPM1G antibody [EPR13362-31]
PPM1G 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13362-31] to PPM1G -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T, Jurkat, HeLa and MCF7 cell lysates; Human kidney clear cell carcinoma and Mouse cardiac muscle tissues; HeLa, 293T and 293 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13362-31 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186423の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
ターゲット情報
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組織特異性
Widely expressed. Most abundant in testis, skeletal muscle, and heart. -
配列類似性
Belongs to the PP2C family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5496 Human
- Entrez Gene: 14208 Mouse
- Entrez Gene: 259229 Rat
- Omim: 605119 Human
- SwissProt: O15355 Human
- SwissProt: Q61074 Mouse
- Unigene: 643951 Human
- Unigene: 14501 Mouse
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別名
- MGC1675 antibody
- MGC2870 antibody
- OTTHUMP00000123426 antibody
see all
画像
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Western blot - Anti-PPM1G antibody [EPR13362-31] (ab186423)All lanes : Anti-PPM1G antibody [EPR13362-31] (ab186423) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219979-anti-ppm1g-antibody-epr13362-31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219978-anti-ppm1g-antibody-epr13362-31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219977-anti-ppm1g-antibody-epr13362-31-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab186423 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (Green). Counter stained with Dapi (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219976-anti-ppm1g-antibody-epr13362-31-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling PPM1G with ab186423 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
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![Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-219975-anti-ppm1g-antibody-epr13362-31-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362-31] (ab186423)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PPM1G with ab186423 at 1/200 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.co.jp/ps/products/186/ab186423/Images/ab186423-7-benefits-of-recombinant-antibodies.png)
Anti-PPM1G antibody [EPR13362-31] (ab186423)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab186423 は論文での使用が確認できていません。