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AB200394

Anti-PPM1F/POPX2 抗体

Anti-PPM1F/POPX2 antibody

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(2 Publications)

Rabbit Polyclonal PPM1F/POPX2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPM1F.

別名を表示する

KIAA0015, POPX2, PPM1F, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase, hFem-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PPM1F/POPX2 with ab200394 at 1/40 dilution.

Western blot - Anti-PPM1F/POPX2 antibody (AB200394)
  • WB

Supplier Data

Western blot - Anti-PPM1F/POPX2 antibody (AB200394)

Gel : 8% SDS-PAGE.

All lanes:

Western blot - Anti-PPM1F/POPX2 antibody (ab200394) at 1/200 dilution

Lane 1:

K562 cell lysate at 40 µg

Lane 2:

Human fetal liver tissue lysate at 40 µg

Predicted band size: 50 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PPM1F. The exact immunogen used to generate this antibody is proprietary information.

P49593

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代替製品

Primary Antibodies

AB236884

Anti-PPM1F/POPX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB236884)

  • 0
  • 0

組合せ製品

Assay Kits

AB283990

Human PPM1F/POPX2 ELISA Kit

Sandwich ELISA - Human PPM1F/POPX2 ELISA Kit (AB283990)

  • 0
  • 0
Primary Antibodies

AB70804

Anti-PPM1B antibody

Immunoprecipitation - Anti-PPM1B antibody (AB70804)

  • 4
  • 1
Primary Antibodies

AB137122

Anti-PPM1E antibody [EPR8721]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (AB137122)

  • 0
  • 0
Primary Antibodies

AB181052

Anti-CaMKII delta antibody [EPR13095]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (AB181052)

  • 5
  • 1
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPM1F also known as POPX2 is a protein phosphatase that belongs to the PP2C family. It functions as a serine/threonine phosphatase and has a molecular mass of approximately 48 kDa. PPM1F is expressed across various tissues including the brain muscle and liver. It plays a role in dephosphorylating proteins involved in numerous cellular processes. By regulating phosphorylation status PPM1F impacts cellular activities such as cell cycle progress and apoptotic pathways.
Biological function summary

This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.

Pathways

Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.

Alterations in PPM1F function have correlations with cancer and neurodegenerative diseases. In cancer PPM1F's dephosphorylation roles impact pathways relevant to cell proliferation and apoptosis contributing to tumor progression. Abnormal regulation of PPM1F has also been observed in certain neurodegenerative disorders where its interactions with proteins such as MAPKs affect neuronal survival and distress responses. The link between PPM1F and these conditions highlights its potential as a therapeutic target in disease management.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
See full target information PPM1F
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105695 PubMed38301894

2024

The BHLHE40‒PPM1F‒AMPK pathway regulates energy metabolism and is associated with the aggressiveness of endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuo Asanoma,Hiroshi Yagi,Ichiro Onoyama,Lin Cui,Emiko Hori,Minoru Kawakami,Shoji Maenohara,Kazuhisa Hachisuga,Hiroshi Tomonobe,Keisuke Kodama,Masafumi Yasunaga,Tatsuhiro Ohgami,Kaoru Okugawa,Hideaki Yahata,Hiroyuki Kitao,Kiyoko Kato

Frontiers in genetics 11:582796 PubMed33193710

2020

Gene and Protein Expression in Subjects With a Nystagmus-Associated AHR Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Borovok,Celeste Weiss,Rajech Sharkia,Michal Reichenstein,Bernd Wissinger,Abdussalam Azem,Muhammad Mahajnah
View all publications
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Abcam product promise

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