AB137023

Anti-PPM1E 抗体 [EPR8722]

Anti-PPM1E antibody [EPR8722]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal PPM1E antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

別名を表示する

CAMKN, KIAA1072, POPX1, PPM1E, Protein phosphatase 1E, Ca(2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-nucleus, Partner of PIX 1, Partner of PIX-alpha, CaMKP-N, CaMKN, Partner of PIXA

2 Images

Flow Cytometry (Intracellular) - Anti-PPM1E antibody [EPR8722] (AB137023)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPM1E antibody [EPR8722] (AB137023)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labelling PPM1E with ab137023 at 1/100 dilution (red). Rabbit IgG negative control is green.

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Western blot - Anti-PPM1E antibody [EPR8722] (AB137023)

All lanes:

Western blot - Anti-PPM1E antibody [EPR8722] (ab137023) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8722

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPM1E also known as protein phosphatase M or POPX1 is a serine/threonine phosphatase with a molecular mass of approximately 82 kDa. It is expressed in various tissues including the brain muscle and heart. PPM1E dephosphorylates its substrates thereby regulating their activities. The enzyme negatively affects phosphorylation-dependent signaling pathways which impacts several biological processes. Its wide expression pattern indicates a broad role in cellular function.

Biological function summary

PPM1E associates with certain complexes that regulate cytoskeletal dynamics and cellular stress responses. It modulates proteins involved in actin cytoskeleton reorganization influencing cell motility and morphology. PPM1E interacts with and regulates targets like CaMKII and PAK which are essential for cell signaling and homeostasis. By acting on these targets PPM1E maintains balance in various cellular activities and processes.

Pathways

PPM1E regulates critical signaling pathways such as the MAPK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK pathway PPM1E regulates signal transduction impacting cellular responses to proliferative signals. It also interacts with calcium/calmodulin-dependent protein kinase modulating calcium signaling which is important for maintaining cardiovascular and neuronal functions. These pathways highlight PPM1E's importance in cellular signaling networks and its interactions with other proteins like MEK and CaMKII.

PPM1E links to neurological disorders and cancer. Changes in PPM1E expression or activity are associated with Alzheimer's disease with its regulatory function influencing proteins like tau an important player in neurodegeneration. In cancer altered PPM1E expression affects cell proliferation and survival often interacting with oncogenic proteins such as RAS. These associations underline the potential of PPM1E as a target for therapeutic strategies in diverse disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).

See full target information PPM1E

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:357 PubMed35436985

2022

Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sandhya Chipurupalli,Raja Ganesan,Giulia Martini,Luigi Mele,Alessio Reggio,Marianna Esposito,Elango Kannan,Vigneshwaran Namasivayam,Paolo Grumati,Vincenzo Desiderio,Nirmal Robinson

Cell research 32:54-71 PubMed34561619

2021

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Xu,Guangjin Ding,Caizhi Liu,Yuhan Ding,Xiaoxin Chen,Xiaoli Huang,Chen-Song Zhang,Shanxin Lu,Yunpeng Zhang,Yuanyong Huang,Zhaosu Chen,Wei Wei,Lujian Liao,Shu-Hai Lin,Jingya Li,Wei Liu,Jiwen Li,Sheng-Cai Lin,Xinran Ma,Jiemin Wong

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPM1E 抗体 [EPR8722]

Anti-PPM1E antibody [EPR8722]

Flow Cytometry (Intracellular) - Anti-PPM1E antibody [EPR8722] (AB137023)

Western blot - Anti-PPM1E antibody [EPR8722] (AB137023)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise