JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248834

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PPM1E antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

別名を表示する

CAMKN, KIAA1072, POPX1, PPM1E, Protein phosphatase 1E, Ca(2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-nucleus, Partner of PIX 1, Partner of PIX-alpha, CaMKP-N, CaMKN, Partner of PIXA

1 Images
Western blot - Anti-PPM1E antibody [EPR8721] - BSA and Azide free (AB248834)
  • WB

Unknown

Western blot - Anti-PPM1E antibody [EPR8721] - BSA and Azide free (AB248834)

This data was developed using ab137122, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPM1E antibody [EPR8721] - BSA and Azide free (ab248834) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Lane 3:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

Observed band size: 84 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8721

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

組合せ製品

Primary Antibodies

AB75854

Anti-UBE2I / UBC9 antibody [EP2938Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-UBE2I / UBC9 antibody [EP2938Y] (AB75854)

  • 4
  • 8
Assay Kits

AB228553

Nile Red Staining Kit

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

  • 0
  • 0
Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB154170

Anti-USP5 antibody [EPR10454]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

ab248834 is the carrier-free version of ab137122.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPM1E also known as protein phosphatase M or POPX1 is a serine/threonine phosphatase with a molecular mass of approximately 82 kDa. It is expressed in various tissues including the brain muscle and heart. PPM1E dephosphorylates its substrates thereby regulating their activities. The enzyme negatively affects phosphorylation-dependent signaling pathways which impacts several biological processes. Its wide expression pattern indicates a broad role in cellular function.
Biological function summary

PPM1E associates with certain complexes that regulate cytoskeletal dynamics and cellular stress responses. It modulates proteins involved in actin cytoskeleton reorganization influencing cell motility and morphology. PPM1E interacts with and regulates targets like CaMKII and PAK which are essential for cell signaling and homeostasis. By acting on these targets PPM1E maintains balance in various cellular activities and processes.

Pathways

PPM1E regulates critical signaling pathways such as the MAPK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK pathway PPM1E regulates signal transduction impacting cellular responses to proliferative signals. It also interacts with calcium/calmodulin-dependent protein kinase modulating calcium signaling which is important for maintaining cardiovascular and neuronal functions. These pathways highlight PPM1E's importance in cellular signaling networks and its interactions with other proteins like MEK and CaMKII.

PPM1E links to neurological disorders and cancer. Changes in PPM1E expression or activity are associated with Alzheimer's disease with its regulatory function influencing proteins like tau an important player in neurodegeneration. In cancer altered PPM1E expression affects cell proliferation and survival often interacting with oncogenic proteins such as RAS. These associations underline the potential of PPM1E as a target for therapeutic strategies in diverse disease contexts.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).
See full target information PPM1E
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com