Anti-PPM1D/WIP1 抗体 (ab31270)
Key features and details
- Rabbit polyclonal to PPM1D/WIP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PPM1D/WIP1 antibody
PPM1D/WIP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPM1D/WIP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Xenopus laevis, Zebrafish
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免疫原
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ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: Jurkat and HEK293 in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31270の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
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機能
Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. -
配列類似性
Belongs to the PP2C family.
Contains 1 PP2C-like domain. - Information by UniProt
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参照データベース
- Entrez Gene: 8493 Human
- Entrez Gene: 53892 Mouse
- Entrez Gene: 380383 Xenopus laevis
- Entrez Gene: 394065 Zebrafish
- Omim: 605100 Human
- SwissProt: O15297 Human
- SwissProt: Q9QZ67 Mouse
- Unigene: 286073 Human
see all -
別名
- EC 3.1.3.16 antibody
- p53 induced protein phosphatase 1 antibody
- p53-induced protein phosphatase 1 antibody
see all
画像
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Western blot - Anti-PPM1D/WIP1 antibody (ab31270)All lanes : Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 20 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab31270 は 11 報の論文で使用されています。
- Li G et al. Wip1 contributes to the adaptation of HepG2 human liver cancer cells to stress hormone-induced DNA damage. Oncol Lett 25:31 (2023). PubMed: 36589663
- Tokheim C et al. Systematic characterization of mutations altering protein degradation in human cancers. Mol Cell 81:1292-1308.e11 (2021). PubMed: 33567269
- Deng K et al. WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC. Signal Transduct Target Ther 5:36 (2020). PubMed: 32296033
- Lu ZW et al. Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma. Oncol Rep 43:783-794 (2020). PubMed: 31922231
- Liu J et al. Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor. Nat Commun 11:4875 (2020). PubMed: 32978388