Anti-PPHLN1/Periphilin-1 抗体 (ab69569)
Key features and details
- Rabbit polyclonal to PPHLN1/Periphilin-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PPHLN1/Periphilin-1 antibody -
製品の詳細
Rabbit polyclonal to PPHLN1/Periphilin-1 -
由来種
Rabbit -
特異性
ab69569 detects endogenous levels of total PPHLN protein. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Extracts from COLO or 293 cells. Liver carcinoma tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
|
| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
-
機能
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation. -
組織特異性
Ubiquitous. -
翻訳後修飾
Substrate of transglutaminase (in vitro). -
細胞内局在
Nucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions. - Information by UniProt
-
参照データベース
- Entrez Gene: 51535 Human
- Entrez Gene: 223828 Mouse
- Omim: 608150 Human
- SwissProt: Q8NEY8 Human
- SwissProt: Q8K2H1 Mouse
- Unigene: 444157 Human
- Unigene: 74208 Mouse
-
別名
- Gastric cancer antigen Ga50 antibody
- Periphilin 1 antibody
- Periphilin-1 antibody
see all
画像
-
Western blot - Anti-PPHLN1/Periphilin-1 antibody (ab69569)All lanes : Anti-PPHLN1/Periphilin-1 antibody (ab69569) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 100 kDa, 35 kDa, 40 kDa, 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPHLN1/Periphilin-1 antibody (ab69569)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab69569 は 5 報の論文で使用されています。
- Das A et al. Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex. J Virol 96:e0203921 (2022). PubMed: 34878926
- Staats K et al. Intermittent parathyroid hormone increases stability and improves osseointegration of initially unstable implants. Bone Joint Res 11:260-269 (2022). PubMed: 35502760
- Douse CH et al. TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control. Nat Commun 11:4940 (2020). PubMed: 33009411
- Zhang F et al. Characterization of a core region in the A2UCOE that confers effective anti-silencing activity. Sci Rep 7:10213 (2017). PubMed: 28860464
- Martrat G et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res 13:R40 (2011). PubMed: 21466675