Anti-PPAR gamma 抗体 (ab45036)
Key features and details
- Rabbit polyclonal to PPAR gamma
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PPAR gamma antibody
PPAR gamma 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPAR gamma -
由来種
Rabbit -
特異性
Ab45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta. This sequence is from P37231-1 (Isoform 2), the sequence is not present in P37231-2 (Isoform 1) or P37231-3 (Isoform 3). -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Pig -
免疫原
Synthetic peptide corresponding to Human PPAR gamma aa 1-100.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45036の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500.
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WB | (2) |
1/500. Predicted molecular weight: 57 kDa.
By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.
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特記事項 |
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ICC/IF
1/500. |
WB
1/500. Predicted molecular weight: 57 kDa. By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.
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ターゲット情報
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機能
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. -
組織特異性
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. -
関連疾患
Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 403606 Dog
- Entrez Gene: 5468 Human
- Entrez Gene: 19016 Mouse
- Entrez Gene: 397671 Pig
- Omim: 601487 Human
- SwissProt: Q4U3Q4 Dog
- SwissProt: P37231 Human
- SwissProt: P37238 Mouse
see all -
別名
- CIMT1 antibody
- GLM1 antibody
- NR1C3 antibody
see all
画像
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Lane 2 : Anti-PPAR gamma antibody (ab45036)
Lane 1 : Protein Marker
Lane 2 : Mouse liver tissue lysate at 1 µg
Secondary
Lane 2 : IRDye® 680RD Donkey anti-Rabbit
Performed under reducing conditions.
Predicted band size: 57 kDa
Exposure time: 2 seconds
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ab45036 staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1:200) for 1 hour at room temperature. A Dylight 680-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/1000).
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All lanes : Anti-PPAR gamma antibody (ab45036) at 1/500 dilution
Lane 1 : 3T3
Lane 2 : 3T3-L1 differentiated day 7
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaPPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.
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ab45036 positive staining PPAR gamma in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.
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Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.
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ab45036 positive staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.
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ab45036 positive staining PPAR gamma in NIH-3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (107)
ab45036 は 107 報の論文で使用されています。
- Jang KO et al. Anti-obesity potential of heat-killed Lactiplantibacillus plantarum K8 in 3T3-L1 cells and high-fat diet mice. Heliyon 9:e12926 (2023). PubMed: 36699277
- Sheng Y et al. Cancer-Associated Fibroblasts Exposed to High-Dose Ionizing Radiation Promote M2 Polarization of Macrophages, Which Induce Radiosensitivity in Cervical Cancer. Cancers (Basel) 15:N/A (2023). PubMed: 36900416
- Yuen JSK et al. Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications. Elife 12:N/A (2023). PubMed: 37014056
- Lin Y et al. Moxibustion ameliorated age-related alterations in neurological behavior and gut microbiota in mice. Am J Transl Res 15:3172-3187 (2023). PubMed: 37303621
- Cao Y et al. ACSM5 inhibits ligamentum flavum hypertrophy by regulating lipid accumulation mediated by FABP4/PPAR signaling pathway. Biol Direct 18:75 (2023). PubMed: 37957699