Anti-PPAR gamma 2 + PPAR gamma 抗体 [A3409A] (ab41928)

Proteins that are found exclusively or predominantly in a sub-cellular location can be enriched in a lysate of the sub-cellular fraction compared to whole cell or tissue lysates. This can be useful when trying to obtain a signal for a weakly-expressed protein. For instance, a nuclear protein will be a larger proportion of the total protein in a nuclear lysate than it will be in a whole-cell or whole-tissue lysate, making it possible to load more of the protein per gel lane. Another advantage is the removal of potentially cross-reactive proteins present in the unused fractions.
For this particular antibody we recommend working with nuclear extracts to optimise the results. The buffer we would use for nuclear extraction is RIPA, but alternatively you could try the following protocol for nuclear fractionation: https://www.abcam.com/index.html?pageconfig=resource&rid=11408.
We also sell sine Cell fractionation kits, which prepare cytosolic, mitochondrial and nuclear fractions in microplate format (see ab109718).
I am afraid we don’t have any further information using this antibody with different conditions, but if you are unable to prepare nuclear lysates you could always try your cellular or tissue whole cell extracts and run a positive control in parallel.
The recommended concentration for using this antibody in WB is 10ug/ml, but it is always advisable to optimise the method as it varies enormously depending on the conditions and samples used.

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Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
Simply follow these easy steps below to apply for our AbTrial Program:
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Please let me know if you have any questions about this offer and I would be happy to help you further.
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If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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