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Anti-PPAR delta 抗体 (ab8937)

Reviews (3)Q&A (2)References (17)
Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab8937)
  • Western blot - Anti-PPAR delta antibody (ab8937)

Key features and details

  • Rabbit polyclonal to PPAR delta
  • Suitable for: ICC/IF, WB, ELISA, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PPAR delta antibody
    PPAR delta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PPAR delta

  • 由来種

    Rabbit

  • 特異性

    This affinity purified antibody is directed against mouse PPAR delta protein. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

  • アプリケーション

    適用あり: ICC/IF, WB, ELISA, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide:

    MEQPQEETPEAREE

    , corresponding to N terminal amino acids 1-14 of Mouse PPAR delta .
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • 3T3 cell lysate HepG2

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8937の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 1 µg/ml.
WB (3)
1/500 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
ELISA
1/30000 - 1/70000.
IHC-P
1/500.
特記事項
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/500 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
ELISA
1/30000 - 1/70000.
IHC-P
1/500.

ターゲット情報

  • 機能

    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.

  • 組織特異性

    Ubiquitous with maximal levels in placenta and skeletal muscle.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FAAR antibody
      • MGC3931 antibody
      • NR1C2 antibody
      • NUC1 antibody
      • NUCI antibody
      • NUCII antibody
      • Nuclear hormone receptor 1 antibody
      • Nuclear receptor subfamily 1 group C member 2 antibody
      • Peroxisome proliferative activated receptor delta antibody
      • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
      • Peroxisome proliferator-activated receptor beta antibody
      • Peroxisome proliferator-activated receptor delta antibody
      • PPAR beta antibody
      • PPAR-beta antibody
      • PPAR-delta antibody
      • PPAR-ß antibody
      • PPARB antibody
      • ppard antibody
      • PPARD_HUMAN antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab8937)
      Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab8937)

      ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-PPAR delta antibody (ab8937)
      Western blot - Anti-PPAR delta antibody (ab8937)

      Rabbit polyclonal to PPAR delta (ab8937).
      Each lane contains 20 ug 3T3 cell lysate

      Lane 1: ab8937 1/500
      Lane 2: ab8937 1/1000

      Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

      Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

    参考文献 (17)

    ab8937 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8937 は 17 報の論文で使用されています。

    • Liu Y  et al. Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ. Nat Commun 13:2665 (2022). PubMed: 35562376
    • Li Y  et al. PPARβ/δ Augments IL-1β-Induced COX-2 Expression and PGE2 Biosynthesis in Human Mesangial Cells via the Activation of SIRT1. Metabolites 12:N/A (2022). PubMed: 35888719
    • Ho SY  et al. Investigating the Role of PPARß/d in Retinal Vascular Remodeling Using Pparß/d-Deficient Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32575793
    • Luo Y  et al. The function of peroxisome proliferator-activated receptors PPAR-? and PPAR-d in Mycobacterium leprae-induced foam cell formation in host macrophages. PLoS Negl Trop Dis 14:e0008850 (2020). PubMed: 33075048
    • Mandai S  et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). PubMed: 29904119
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-PPAR delta antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Colorectal cancer HCT116)
    Loading amount
    10 µg
    Specification
    Colorectal cancer HCT116
    Gel Running Conditions
    Non-reduced Denaturing (10% Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Injoo Hwang

    Verified customer

    投稿 Nov 09 2009

    Western blot abreview for Anti-PPAR delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (Liver)
    Loading amount
    20 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 10 2009

    Western blot abreview for Anti-PPAR delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Loading amount
    30 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 07 2008

    Question

    AbCam Technical Support: I have written the answers below in CAPITAL letters beneath each issue. Thanks for any help you can provide > Also, please include the lot number (located on your vial) 68633 and the Abcam order number or purchase order number that was used. 1. Please describe the problem (high background, wrong band size, more bands, no band etc). THE PROBLEM IS THAT THE BAND THAT STAINS MOST INTENSELY IS LOCATED AROUND 70-75 kDA. MULITPLE OTHER BANDS APPEAR WITH LONGER EXPOSURE TIMES AND IT IS UNCLEAR WHICH BAND, IF ANY, REPRESENTS PPAR b. 2. On what material are you testing the antibody in WB? Species? HUMAN Cell extract/ Nuclear extract? CYTOPLASMIC AND NUCLEAR PROTEIN EXTRACTS FROM TWO DIFFERENT HUMAN CELL LINES Purified protein? NO Recombinant protein? NO 3. How much protein did you load? 34 ug/WELL (SEB-1 CELL LINE) AND 70 ug/WELL (HaCaT CELL LINE) I COULD NOT DETERMINE A DIFFERENCE BETWEEN THESE TWO CONCENTRATIONS ON THE BLOT How did you prepare the lysate for the analysis (protease inhibitors) SAMPLES WERE PREPARED USING NE-PER NUCLEAR AND CYTOPLASMIC EXTRACTION REAGENTS KIT FROM PIERCE AS DIRECTED IN THE MANUFACTURERS PROTOCOL. PROTEASE INHIBITORS WERE ADDED. Did you heat the samples? SAMPLES WERE HEATED TO 70º C FOR 10 MINUTES PRIOR TO LOADING ON GEL. 4. Primary Antibody ABCAM PPAR DELTA AB 8937 Specification (in which species was it raised against)? RABBIT POLYCLONAL At what dilution(s) have you tested this antibody? 1:1000 Incubation time, wash step? INCUBATED OVERNIGHT AT 4º C AND THEN WASHED 3 x 5 MINUTES IN TBS-T (0.05% TWEEN) 5. Secondary Antibody ANTI-RABBIT HRP FROM CELL SIGNALLING TECHNOLOGY. Specification (in which species was it raised against)? RABBIT At what dilution(s) have you tested this antibody? 1:2000 Incubation time, wash step? INCUABTED FOR 1 HOUR AT ROOM TEMPERATURE IN 5% DRY MILK, TWEEN-20 (0.05%) AND THEN WASHED 2 X 5 MINUTES IN TBS-T AND 1 X 5 MINUTES IN TBS Do you know whether the problems you are experiencing come from the secondary? SECONDARY ANTIBODY HAS BEEN USED NUMEROUS TIMES WITHOUT A PROBLEM 6. What detection method are you using? SUPERSIGNAL WEST PICO CHEMILUMINENCENT SUBSTRATE KIT FROM PIERCE AS DIRECTED. 7. Background bands Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a â??No primaryâ?? control) YES AND LANE IS CLEAR OF BANDS Is the blocking step sufficient? (We recommend blocking the membrane by adding 20 ml of blocking buffer (5% non-fat dry milk, 0.1% Tween-20 in TBS). Incubate for 2 h at room temperature or overnight at 4°C with agitation) BLOCKING FOR 1 HOUR HAS ALWAYS BEEN SUFFICENT FOR US. Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) WASHING 3 X 5 MINUTES AS IS STANDARD IN MOST PROTOCOLS At what size are the bands migrating? Could they be degradation products of your target? THE MAJOR BAND IN QUESTION IS THE ONE THAT MIGRATES AROUND 70-75 kDA, WHY IS THIS ONE MORE INTENSE THAN THE OTEHRS??? WHICH BAND IS THE CORRECT ONE FOR PPAR DELTA???????? Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient) 8. Optimization attempts How many times have you tried the Western? NUMEROUS TIMES WITH OTHER COMPANY ANTIBODY AND NEVER GOTTEN RESULTS LIKE THIS. BLOT IS FREE OF BACKGROUND JUST BANDS IN WRONG PLACE. Do you obtain the same results every time e.g. are background bands always in the same place? N/A What steps have you altered? N/A 9. Did you apply positive and negative controls along with the samples? Please specify. NEGATIVE CONTROL WAS PRIMARY ANTIBODY ALONE. WE DID NOT RUN ANY SAMPLES AS POSITIVE OR NEGATIVE CONTROLS.

    Read More
    Answer

    Thank you for the details that you have provided us with. This antibody has been characterized in Western blotting using 3T3 cell lysate, and we have not specifically tested it with human samples (although it is expected to cross-react due to sequence homology). So I would strongly suggest running a positive control, such as 3T3 lysate. We saw a band at approximately 53 kDa using ab8937 with 3T3 lysate. I was unfortunately unable to open the attachment that you sent, but to try to get reduce the number of multiple bands that you are seeing, you may want to decrease the concentrations of both the primary and secondary antibodies. However, in your case the one thing that I think you need to do is run a positive control. If you need further assistance, please contact us again.

    Read More

    Question

    I would like to know if you offer an ELISA kit for this Antibody. Thank-you very much in advance

    Read More
    Answer

    Thank you for your enquiry regarding ab8937. Unfortunately we don't supply Elisa Kits for this antibody. In the future, if you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). Should you require any further information to that shown on the datasheets, please get in touch with us and we will gladly assist you.

    Read More

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