AB8937

Anti-PPAR delta 抗体

Name: Anti-PPAR delta 抗体 (ab8937) | Abcam
Brand: Abcam Limited
SKU: AB8937
Price: 77000 JPY
Availability: InStock
Rating: 4 (3 reviews)

Anti-PPAR delta antibody

(3 Reviews)

(20 Publications)

Rabbit Polyclonal PPAR delta antibody. Suitable for WB, ICC/IF, ELISA, IHC-P and reacts with Mouse, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ppard aa 1-50.

別名を表示する

NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (AB8937)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (AB8937)

ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Western blot - Anti-PPAR delta antibody (AB8937)

Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysate

Lane 1 : ab8937 1/500
Lane 2 : ab8937 1/1000

Secondary antibody : Goat anti-Rabbit (HRP) (ab6721) at 1/2000

Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1 : ab8937 1/500 Lane 2 : ab8937 1/1000 Secondary antibody : Goat anti-Rabbit (HRP) (ab6721) at 1/2000

All lanes:

Western blot - Anti-PPAR delta antibody (ab8937)

Predicted band size: 50 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, ELISA, IHC-P, WB

applications

免疫原

Synthetic Peptide within Mouse Ppard aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35396

特異性

This affinity purified antibody is directed against mouse PPAR delta protein. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/30000 - 1/70000", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/30000 - 1/70000", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed : 35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.

See full target information PPARD

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110046 PubMed38989454

2024

PPARdelta: A key modulator in the pathogenesis of diabetes mellitus and co-morbidity.

Applications

Unspecified application

Species

Unspecified reactive species

Halemah AlSaeed,Mohammed J A Haider,Fawaz Alzaid,Fahd Al-Mulla,Rasheed Ahmad,Fatema Al-Rashed

iScience 26:107145 PubMed37416456

2023

ACSL1 is a key regulator of inflammatory and macrophage foaming induced by short-term palmitate exposure or acute high-fat feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Fatema Al-Rashed,Dania Haddad,Ashraf Al Madhoun,Sardar Sindhu,Texy Jacob,Shihab Kochumon,Lina M Obeid,Fahd Al-Mulla,Yusuf A Hannun,Rasheed Ahmad

Oncology research 30:289-300 PubMed37303493

2023

LSM12 facilitates the progression of colorectal cancer by activating the WNT/CTNNB1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhuang,Chunlan Ning,Pengfei Liu,Yanpeng Zhao,Yue Li,Zhenchi Ma,Luling Shan,Yingzhe Piao,Peng Zhao,Xun Jin

Metabolites 12: PubMed35888719

2022

PPARβ/δ Augments IL-1β-Induced COX-2 Expression and PGE2 Biosynthesis in Human Mesangial Cells via the Activation of SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqing Li,Rong Cao,Tingting Gu,Cong Cao,Tingyue Chen,Youfei Guan,Xiaoyan Zhang

Nature communications 13:2665 PubMed35562376

2022

Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Yasunori Deguchi,Daoyan Wei,Fuyao Liu,Micheline J Moussalli,Eriko Deguchi,Donghui Li,Huamin Wang,Lovie Ann Valentin,Jennifer K Colby,Jing Wang,Xiaofeng Zheng,Haoqiang Ying,Mihai Gagea,Baoan Ji,Jiaqi Shi,James C Yao,Xiangsheng Zuo,Imad Shureiqi

PLoS neglected tropical diseases 14:e0008850 PubMed33075048

2020

The function of peroxisome proliferator-activated receptors PPAR-γ and PPAR-δ in Mycobacterium leprae-induced foam cell formation in host macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqian Luo,Kazunari Tanigawa,Akira Kawashima,Yuko Ishido,Norihisa Ishii,Koichi Suzuki

International journal of molecular sciences 21: PubMed32575793

2020

Investigating the Role of PPARβ/δ in Retinal Vascular Remodeling Using /-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sze Yuan Ho,Yuet Ping Kwan,Beiying Qiu,Alison Tan,Hannah Louise Murray,Veluchamy Amutha Barathi,Nguan Soon Tan,Chui Ming Gemmy Cheung,Tien Yin Wong,Walter Wahli,Xiaomeng Wang

Scientific reports 8:9101 PubMed29904119

2018

WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Mandai,Takayasu Mori,Naohiro Nomura,Taisuke Furusho,Yohei Arai,Hiroaki Kikuchi,Emi Sasaki,Eisei Sohara,Tatemitsu Rai,Shinichi Uchida

Cellular physiology and biochemistry : internation 47:545-555 PubMed29794473

2018

MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL.

Applications

Unspecified application

Species

Unspecified reactive species

Xumin Hu,Jianhua Tang,Xuyun Hu,Peng Bao,Jinxin Pan,Zhipeng Chen,Jiaqi Xian

Cell transplantation 26:1405-1417 PubMed28901183

2017

PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial Fluid.

Applications

Species

Human

Bruce E Heck,Joshua J Park,Vishruti Makani,Eun-Cheol Kim,Dong Hyun Kim

View all publications

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Abcam product promise

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Anti-PPAR delta 抗体

Anti-PPAR delta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (AB8937)

Western blot - Anti-PPAR delta antibody (AB8937)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (20)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise